EZH2/KMT6 Antibody

Images

 
Western Blot: KMT6/EZH2 Antibody [NBP2-17087] - Sample (30 ug of whole cell lysate) A: H1299 B: HCT116 C: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: EZH2/KMT6 Antibody [NBP2-17087] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EZH2 protein stained by EZH2 antibody diluted at 1:500. Red: phalloidin, ...read more
Chromatin Immunoprecipitation: EZH2/KMT6 Antibody [NBP2-17087] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-EZH2 antibody. The precipitated DNA was detected ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Fi, Mk, Xp, ZeSpecies Glossary
Applications WB, ChIP, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

EZH2/KMT6 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EZH2. The exact sequence is proprietary.
Predicted Species
Mouse (99%), Rat (99%), Porcine (99%), Monkey (100%), Xenopus (97%), Zebrafish (94%), Bovine (99%), Fish (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EZH2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunoprecipitation
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (100%), Xenopus laevis (97%), Japanese medaka (95%),

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EZH2/KMT6 Antibody

  • EC 2.1.1
  • EC 2.1.1.43
  • enhancer of zeste (Drosophila) homolog 2
  • enhancer of zeste 2
  • enhancer of zeste homolog 2 (Drosophila)
  • Enhancer of zeste homolog 2
  • ENX1
  • ENX-1KMT6EZH1KMT6A
  • EZH2
  • histone-lysine N-methyltransferase EZH2
  • KMT6
  • Lysine N-methyltransferase 6
  • MGC9169

Background

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EZH2
Entrez
Uniprot