EZH1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: EZH1 Antibody [NBP1-32111] - Paraformaldehyde-fixed HeLa, using EZH1 antibody at 1:1000 dilution.

Immunohistochemistry-Paraffin: EZH1 Antibody [NBP1-32111] - H1299 xenograft, using EZH1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: EZH1 Antibody [NBP1-32111] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EZH1 antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EZH1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit EZH1 Antibody - BSA Free (NBP1-32111) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human EZH1. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EZH1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for EZH1 Antibody - BSA Free

• EC 2.1.1.356
• EC 2.1.1.43
• enhancer of zeste homolog 1 (Drosophila)
• Enhancer of zeste homolog 1
• ENX-2
• EZH1
• histone-lysine N-methyltransferase EZH1
• KIAA0388
• KIAA0388enhancer of zeste (Drosophila) homolog 1
• KMT6B

Background

EZH1 is a human homolog of the Drosophila gene Enhancer of zeste E(z), a member of the polycomb group of transcriptional repressors. In mice, EZH1 is a regulator of homeotic gene expression implicated in the assembly of repressive protein complexes in chromatin. The strong sequence conservation between species suggests potential role for EZH1 in human development as a transcriptional regulator and as a component of protein complexes that stably maintain heterochromatin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Review for EZH1 Antibody (NBP1-32111) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Alex Schmidt WB Human 11/25/2014

Summary

• Application: Western Blot
• Sample Tested: HeLa cells
• Species: Human

Bioinformatics

• Gene Symbol: EZH1