EZH1 Antibody

Images

 
Western Blot: EZH1 Antibody [NBP1-32111] - Sample(30 ug of whole cell lysate)A:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: EZH1 Antibody [NBP1-32111] - Paraformaldehyde-fixed HeLa, using EZH1 antibody at 1:1000 dilution.
Immunohistochemistry-Paraffin: EZH1 Antibody [NBP1-32111] - Paraffin-embedded H1299 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

EZH1 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human EZH1. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EZH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-32111 in the following applications:

Reactivity Notes

Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EZH1 Antibody

  • EC 2.1.1.43
  • enhancer of zeste homolog 1 (Drosophila)
  • Enhancer of zeste homolog 1
  • ENX-2
  • histone-lysine N-methyltransferase EZH1
  • KIAA0388enhancer of zeste (Drosophila) homolog 1
  • KMT6B

Background

EZH1 is a human homolog of the Drosophila gene Enhancer of zeste E(z), a member of the polycomb group of transcriptional repressors. In mice, EZH1 is a regulator of homeotic gene expression implicated in the assembly of repressive protein complexes in chromatin. The strong sequence conservation between species suggests potential role for EZH1 in human development as a transcriptional regulator and as a component of protein complexes that stably maintain heterochromatin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EZH1 Antibody (NBP1-32111) (0)

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Review for EZH1 Antibody (NBP1-32111) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-32111:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot EZH1 NBP1-32111
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reviewed by:
Alex Schmidt
WB Human 11/25/2014
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Summary

ApplicationWestern Blot
Sample TestedHeLa cells
SpeciesHuman

Blocking

Blocking Details4 % BSA in TBST

Primary Anitbody

Dilution RatioDiluted 1:1000 times

Secondary Antibody

Secondary DescriptionGoat Anti-Rabbit IgG HRP Affinity Purified PAb (R&D Systems)
Secondary Manufacturer Cat#HAF007
Secondary ConcentrationDilution: 10,000 times

Details

Detection NotesOne-hour Western detection system (Genscript), 30 sec exposure

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EZH1 Antibody (NBP1-32111) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for EZH1 Antibody (NBP1-32111)

Discover related pathways, diseases and genes to EZH1 Antibody (NBP1-32111). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for EZH1 Antibody (NBP1-32111)

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Blogs on EZH1.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

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Recent Reviews

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4
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1
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Alex Schmidt
11/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol EZH1