EWSR1 Antibody (5C10)

Western Blot: EWSR1 Antibody (5C10) [H00002130-M01] - Analysis of EWSR1 expression in transfected 293T cell line by EWSR1 monoclonal antibody (M01), clone 5C10.Lane 1: EWSR1 transfected lysate(68.5 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: EWSR1 Antibody (5C10) [H00002130-M01] - Analysis of monoclonal antibody to EWSR1 on formalin-fixed paraffin-embedded human urinary bladder. Antibody concentration 3 ug/ml.

ELISA: EWSR1 Antibody (5C10) [H00002130-M01] - Detection limit for recombinant GST tagged EWSR1 is approximately 0.03ng/ml as a capture antibody.

Product Discontinued

EWSR1 Antibody (5C10) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: EWSR1 (NP_005234, 358 a.a. ~ 453 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SDNSAIYVQGLNDSVTLDDLADFFKQCGVVKMNKRTGQPMIHIYLDKETGKPKGDATVSYEDPPTAKAAVEWFDGKDFQGSKLKVSLARKKPPMNS
• Specificity: EWSR1 - Ewing sarcoma breakpoint region 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EWSR1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EWSR1 Antibody (5C10)

• bK984G1.4
• Ewing sarcoma breakpoint region 1 protein
• Ewing sarcoma breakpoint region 1
• Ewings sarcoma EWS-Fli1 (type 1) oncogene
• EWS oncogene
• EWS
• EWSR1
• EWSRNA-binding protein EWS

Background

This gene encodes a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EWSR1
• Entrez:
  • 2130(Human)
• OMIM:
  • 133450(Human)
• Uniprot:
  • NP_005234(Human)