ETFB Antibody - BSA Free

Western Blot: ETFB Antibody [NBP2-97524] - Anti-ETFB rabbit polyclonal antibody at 1:500 dilution. Lane A: MOLT4 Whole Cell Lysate Lane B: Mouse liver tissue lysate Lane C: U-251 MG Whole Cell Lysate Lane D: NIH-3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 27 kDa

Immunocytochemistry/Immunofluorescence: ETFB Antibody [NBP2-97524] - Immunofluorescence staining of ETFB in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ETFB polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunohistochemistry-Paraffin: ETFB Antibody [NBP2-97524] - Immunochemical staining of human ETFB in human liver with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: ETFB Antibody [NBP2-97524] - Immunochemical staining of human ETFB in cynomolgus heart with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ETFB Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human ETFB fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ETFB
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for ETFB Antibody - BSA Free

• beta-ETF
• electron transfer flavoprotein beta subunit
• electron transfer flavoprotein beta-subunit
• electron transfer flavoprotein subunit beta
• electron transfer flavoprotein, beta polypeptide
• electron-transfer-flavoprotein, beta polypeptide
• electron-transferring-flavoprotein, beta polypeptide
• MADD

Background

ETFB is a is an electron-transfer-flavoprotein, (beta polypeptide), and a subcomponent of Electron transfer flavoprotein (ETF). ETF exists in the mitochondrial matrix as a heterodimer of 30-kD alpha subunits (ETFA) and 28-kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer. This electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases and transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ETFB