Erythropoietin/EPO Antibody [Unconjugated]

Recombinant Mouse Erythropoietin/EPO (Catalog #959‑ME) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Erythropoietin/EPO (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Erythropoietin/EPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF959). The ND50 is typically 0.25‑1.25 µg/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Erythropoietin/EPO Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Erythropoietin/EPO
  Ala27-Arg192
  Accession # P07321
• Specificity: Detects human, mouse, and rat Erythropoietin/EPO in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Thymopoietin (Tpo) is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EPO
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.25-1.25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Erythropoietin/EPO Antibody [Unconjugated]

• ECYT5
• EP
• EPO
• epoetin
• Erythropoietin
• MGC138142
• MVCD2

Background

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of EPO is required for biological activities in vivo (4). Mature mouse EPO shares 95% amino acid sequence identity with rat EPO and 73%-82% with bovine, canine, equine, feline, human, ovine, and porcine EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6-8). EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R) (8, 9). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).

1. Koury, M. J. (2005) Exp. Hematol. 33:1263.
2. Shoemaker, C.B. and L.D. Mitsock (1986) Mol. Cell. Biol 6:849.
3. Wen, D. et al. (1993) Blood 82:1507.
4. Tsuda E. et al. (1990) Eur. J. Biochem. 188:405.
5. Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
6. Eckardt, K. U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
7. Sharples, E. J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
8. Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
9. Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
10. Acs, G. et al. (2001) Cancer Res. 61:3561.
11. Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
12. Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
13. Kertesz, N. et al. (2004) Dev. Biol. 276:101.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Erythropoietin/EPO Antibody (AF959)(7)

Publications using AF959

• Hosaka K, Wang C, Zhang S et al. Perivascular localized cells commit erythropoiesis in PDGF-B-expressing solid tumors Cancer communications (London, England) 2023-04-29 [PMID: 37120719]
• V Brizi, S Buttò, D Cerullo, A Michele La, R Rodrigues-, R Novelli, D Corna, A Benigni, G Remuzzi, C Xinaris Human iPSC-derived neural crest stem cells can produce EPO and induce erythropoiesis in anemic mice Stem Cell Research, 2021-07-25;55(0):102476. 2021-07-25 [PMID: 34339993] (Neutralization, Human)
• K Toriuchi, H Kakita, T Tamura, S Takeshita, Y Yamada, M Aoyama Prolonged astrocyte-derived erythropoietin expression attenuates neuronal damage under hypothermic conditions J Neuroinflammation, 2020-05-02;17(1):141. 2020-05-02 [PMID: 32359362] (Cell Culture, Rat)
• Muhammed A. Saad, Ayman E. El-Sahar, Rabab H. Sayed, Eman M. Elbaz, Hebatullah S. Helmy, Mahmoud A. Senousy Venlafaxine Mitigates Depressive-Like Behavior in Ovariectomized Rats by Activating the EPO/EPOR/JAK2 Signaling Pathway and Increasing the Serum Estradiol Level Neurotherapeutics 2019-04-01 [PMID: 30361931]
• YH Chen, HL Feng, SS Jeng Zinc Supplementation Stimulates Red Blood Cell Formation in Rats Int J Mol Sci, 2018-09-18;19(9):. 2018-09-18 [PMID: 30231592] (Neutralization, Rat)
• Heather H. Ross, Milap S. Sandhu, Tina F. Cheung, Garrett M. Fitzpatrick, Warren J. Sher, Alexander J. Tiemeier et al. In vivo intermittent hypoxia elicits enhanced expansion and neuronal differentiation in cultured neural progenitors Experimental Neurology 2012-05-01 [PMID: 22366327]
• Pak M, Lopez MA, Gabayan V, Ganz T, Rivera S Suppression of hepcidin during anemia requires erythropoietic activity. Blood, 2006-08-01;108(12):3730-5. 2006-08-01 [PMID: 16882706] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: EPO
• Uniprot:
  • P07321()