Erythropoietin/EPO Antibody (9C21D11) [Unconjugated]

Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (Catalog # 287-TC) stimulates proliferation in theTF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.3 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB287). The ND50 is typically 0.5‑1.5 µg/mL.

• Reactivity: Hu
• Applications: B/N
• Clone: 9C21D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Erythropoietin/EPO Antibody (9C21D11) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Erythropoietin/EPO
  Accession # P01588
• Specificity: Detects human Erythropoietin/EPO.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPO
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.5-1.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Erythropoietin/EPO Antibody (9C21D11) [Unconjugated]

• ECYT5
• EP
• EPO
• epoetin
• Erythropoietin
• MGC138142
• MVCD2

Background

Erythropoietin (EPO) a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Erythropoietin/EPO Antibody (MAB287)(10)

Publications using MAB287

• A Costello, NT Lao, N Barron, M Clynes Continuous Translation of Circularized mRNA Improves Recombinant Protein Titer Metab. Eng., 2019-01-04;0(0):. 2019-01-04 [PMID: 30615942] (ELISA Development (Capture), Transgenic Hamster)
• R Maldonado-, AJ Dickson Multiplexed digital mRNA expression analysis profiles system-wide changes in mRNA abundance and responsiveness of UPR-specific gene expression changes during batch culture of recombinant Chinese Hamster Ovary cells Biotechnol J, 2018-02-02;0(0):. 2/2/2018 [PMID: 29323465]
• H Zhang, X Fang, D Huang, Q Luo, M Zheng, K Wang, L Cao, Z Yin Erythropoietin signaling increases neurogenesis and oligodendrogenesis of endogenous neural stem cells following spinal cord injury both in�vivo and in�vitro Mol Med Rep, 2017-10-25;0(0):. 10/25/2017 [PMID: 29115443]
• Mirko Pesce, Paolo Felaco, Sara Franceschelli, Lorenza Speranza, Alfredo Grilli, Maria Anna Anna De Lutiis et al. Effect of erythropoietin on primed leucocyte expression profile Open Biology 2014-06-13 [PMID: 24920275]
• Fliedl L, Manhart G, Kast F, Katinger H, Kunert R, Grillari J, Wieser M, Grillari-Voglauer R Novel human renal proximal tubular cell line for the production of complex proteins. J Biotechnol, 2014-02-16;176(0):29-39. 2014-02-16 [PMID: 24553072] (ELISA Development (Capture), Human)
• Séverine Lamon, Aaron P. Russell The role and regulation of erythropoietin (EPO) and its receptor in skeletal muscle: how much do we really know? Frontiers in Physiology 2013-07-15 [PMID: 23874302]
• Lilach Lifshitz, Galit Tabak, Max Gassmann, Moshe Mittelman, Drorit Neumann Macrophages as novel target cells for erythropoietin Haematologica 2010-11-01 [PMID: 20595096]
• Kondo A, Shingo T, Yasuhara T, Kuramoto S, Kameda M, Kikuchi Y, Matsui T, Miyoshi Y, Agari T, Borlongan CV, Date I Erythropoietin exerts anti-epileptic effects with the suppression of aberrant new cell formation in the dentate gyrus and upregulation of neuropeptide Y in seizure model of rats. Brain Res., 2009-08-18;1296(0):127-36. 2009-08-18 [PMID: 19695235] (Neutralization, Rat)
• MacRedmond R, Singhera GK, Dorscheid DR Erythropoietin inhibits respiratory epithelial cell apoptosis in a model of acute lung injury. Eur. Respir. J., 2009-01-22;33(6):1403-14. 2009-01-22 [PMID: 19164355] (Neutralization, Human)
• Kirkeby A, van Beek J, Nielsen J, Leist M, Helboe L Functional and immunochemical characterisation of different antibodies against the erythropoietin receptor. J Neurosci Methods, 2007-04-12;164(1):50-8. 2007-04-12 [PMID: 17524492] (ICC, Western Blot, Human)

Bioinformatics

• Gene Symbol: EPO
• Uniprot:
  • P01588()