ERR gamma/NR3B3 Antibody

Immunohistochemistry-Paraffin: ERR gamma/NR3B3 Antibody [NB100-59007] - Analysis of anti-ESRRG / ERR3 antibody with human kidney at concentration 10 ug/ml.

Product Discontinued

ERR gamma/NR3B3 Antibody Summary

• Immunogen: Synthetic 15 amino acid peptide from N-terminus of human ERR gamma/NR3B3.
• Epitope: N-terminal domain of human
• Specificity: Human ESRRG / ERR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ESRRG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
• Control:

  ERR gamma/NR3B3 Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Opossum (87%), Chicken (87%), Xenopus (87%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ERR gamma/NR3B3 Antibody

• DKFZp781L1617
• ERR gamma
• ERR gamma-2
• ERR3
• ERRG2
• ERRgamma
• ESRRG
• Estrogen receptor-related protein 3
• estrogen-related receptor gamma
• FLJ16023
• KIAA0832
• NR3B3
• Nuclear receptor subfamily 3 group B member 3

Background

Estrogen related receptor (ERR gamma), a NR3 Steroid Receptor is an orphan member of the nuclear hormone receptor superfamily. All the ERR family members (1,2, and 3) share an almost identical DNA binding domain, which has 68% amino acid identity with that of estrogen receptor. Studies involving the crystal structure of the LBD have shown a complex between ERR3 and SRC1. ERR gamma has been suggested to affect differentiation of the brain, heart, and kidney. ERR gamma binds as a monomer to an extended half site of the ERRE type (TCAAGGTCA). ERR gamma has been shown to interact with PGC1 alpha and has been implicated in the regulation of mitochondrial energy metabolism. In humans, ERR gamma pre mRNA undergoes extensive alternative splicing at the 5 prime end, yielding at least six mRNA splice variants and two protein isoforms that differ by 23 amino acids in the N terminus. ERR gamma has been shown to be over expressed in breast tumors, and its expression is correlated with levels of ErbB4, a likely indicator of preferred clinical course. As a result, ERR gamma has been suggested to be a potential biomarker for favorable clinical course and, possibly, hormonal sensitivity, and as a candidate target for therapeutic development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESRRG
• Entrez:
  • 2104(Human)