ERP29 Overexpression Lysate (Native)

Images

 
Western Blot: ERP29 Overexpression Lysate (Adult Normal) [NBL1-10338] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ERP29.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ERP29 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210918 and based on accession number NM_006817. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens endoplasmic reticulum protein 29 (ERP29), transcript variant 1, mRNA.
Gene
ERP29

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
25.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ERP29

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ERP29 Overexpression Lysate (Native)

  • C12orf8chromosome 12 open reading frame 8
  • endoplasmic reticulum lumenal protein ERp28
  • endoplasmic reticulum protein 29
  • Endoplasmic reticulum resident protein 28
  • endoplasmic reticulum resident protein 29
  • ERp28Endoplasmic reticulum resident protein 31
  • ERp29
  • ERp31ERP28
  • PDIA9
  • PDI-DB
  • protein disulfide isomerase family A, member 9

Background

This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for ERP29 Lysate (NBL1-10338) (0)

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Bioinformatics

Gene Symbol ERP29