ERP29 Antibody (JG34-42)

Western Blot: ERP29 Antibody (JG34-42) [NBP2-75483] - Analysis of ERp29 on THP-1 cell using anti-ERp29 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: ERP29 Antibody (JG34-42) [NBP2-75483] - Staining ERp29 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ERP29 Antibody (JG34-42) [NBP2-75483] - Analysis of paraffin-embedded rat testis tissue using anti-ERp29 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: ERP29 Antibody (JG34-42) [NBP2-75483] - Staining ERp29 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: ERP29 Antibody (JG34-42) [NBP2-75483] - Staining ERp29 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ERP29 Antibody (JG34-42) [NBP2-75483] - Analysis of paraffin-embedded human thyroid gland tissue using anti-ERp29 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ERP29 Antibody (JG34-42) [NBP2-75483] - Analysis of paraffin-embedded human colon cancer tissue using anti-ERp29 antibody. Counter stained with hematoxylin.

Product Discontinued

ERP29 Antibody (JG34-42) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human ERP29 aa 1-142 / 261. (SwissProt: P30040 Human; SwissProt: P57759 Mouse; SwissProt: P52555 Rat)
• Localization: Endoplasmic reticulum.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ERP29
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ERP29 Antibody (JG34-42)

• C12orf8chromosome 12 open reading frame 8
• endoplasmic reticulum lumenal protein ERp28
• endoplasmic reticulum protein 29
• Endoplasmic reticulum resident protein 28
• endoplasmic reticulum resident protein 29
• ERp28Endoplasmic reticulum resident protein 31
• ERp29
• ERp31ERP28
• PDIA9
• PDI-DB
• protein disulfide isomerase family A, member 9

Background

ERP29 encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERP29