ERLIN1 Antibody - Azide and BSA Free

Western Blot: ERLIN1 Antibody [NBP3-04903] - Analysis of extracts of various cell lines, using ERLIN1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit

Immunohistochemistry-Paraffin: ERLIN1 Antibody [NBP3-04903] - Immunohistochemistry of paraffin-embedded human colon using ERLIN1 antibody (NBP3-04903) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunoprecipitation: ERLIN1 Antibody - Azide and BSA Free [ERLIN1] - Immunoprecipitation analysis of 300 ug extracts of DU145 cells using 3 ug ERLIN1 antibody . Western blot was performed from the immunoprecipitate using ERLIN1 antibody at a dilution of 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ERLIN1 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit ERLIN1 Antibody - Azide and BSA Free (NBP3-04903) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 259-348 of human ERLIN1 (NP_006450.2). KADAEYYAAHKYATSNKHKLTPEYLELKKYQAIASNSKIYFGSNIPNMFVDSSCALKYSDIRTGRESSLPSKEALEPSGENVIQNKESTG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ERLIN1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:500 - 1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for ERLIN1 Antibody - Azide and BSA Free

• Band_7 23-211 Keo4 (Interim) similar to C.elegans protein C42C1.9
• C10orf69
• chromosome 10 open reading frame 69
• Endoplasmic reticulum lipid raft-associated protein 1
• ER lipid raft associated 1
• Erlin-1
• KE04SPFH domain-containing protein 1
• KEO4
• Protein KE04
• SPFH domain family, member 1
• SPFH1
• Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 1

Background

ERLIN1 is a component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: ERLIN1