ERK1/ERK2 Antibody (SR1700) - BSA Free

Western Blot: ERK1/ERK2 Antibody (SR1700) [NBP3-21670] - Western blot analysis using NBP3-21670 in HepG2 whole cell lysates.

Immunocytochemistry/Immunofluorescence: ERK1/ERK2 Antibody (SR1700) [NBP3-21670] - Immunofluorescent analysis of Hela cells, using NBP3-21670.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, Flow, ICC/IF, IP
• Clone: SR1700
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ERK1/ERK2 Antibody (SR1700) - BSA Free Summary

• Description: Novus Biologicals Rabbit ERK1/ERK2 Antibody (SR1700) - BSA Free (NBP3-21670) is a recombinant monoclonal antibody validated for use in WB, Flow, ICC/IF, Simple Western and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human ERK1/ERK2 (Uniprot #: P27361, P28482)
• Specificity: Detects endogenous levels of total ERK1/ERK2
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAPK3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation 1:50
  • Simple Western 1:10 - 1:250
  • Western Blot 1:500-1:2000
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Tested in PDLO lysate, separated by Size, antibody dilution of 1:10, 1:50, 1:250, apparent MW was 42 kDa

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ERK1/ERK2 Antibody (SR1700) - BSA Free

• ERK1
• ERK-1
• ERK1/ERK2
• ERT2
• Extracellular signal-regulated kinase 1
• extracellular signal-related kinase 1
• HS44KDAP
• HUMKER1A
• Insulin-stimulated MAP2 kinase
• MAPK 1
• Microtubule-associated protein 2 kinase
• mitogen-activated protein kinase 3
• p44erk1
• p44-ERK1
• p44mapk
• p44-MAPK
• PRKM3

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK3