ERK1 Antibody - BSA Free

Western Blot: ERK1 Antibody [NBP1-78031] - Detection of ERK1 in whole cell lysates prepared from mouse brain (left), mouse kidney (center) and mouse intestine (right).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ERK1 Antibody - BSA Free Summary

• Description: This affinity-purified antibody is directed against the human ERK1 (ERK1) protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified ERK1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal end of human p44 ERK1 protein. (Uniprot: P27361)
• Specificity: This affinity-purified antibody is directed against the human p44 ERK1 protein. A BLAST analysis was used to suggest cross reactivity with p44 ERK1 proteins from human, bovine, rabbit, rat and mouse. No reactivity is observed against p42 MAP Kinase (ERK2). Reactivity against homologues from other sources is not known.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000-1:10000
  • Immunoprecipitation 5.0 ug/mg
  • Western Blot 1:1000-1:2000
• Application Notes: This affinity purified antibody has been tested for use in ELISA, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 44 kDa in size corresponding to p44 MAP Kinase (ERK1) by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross reactivity with p44 ERK1 proteins from human, bovine, rabbit, rat and mouse. No reactivity is observed against p42 MAP Kinase (ERK2). Reactivity against homologues from other sources is not known.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ERK1 Antibody - BSA Free

• EC 2.7.11
• ERK1
• ERK-1
• ERK1p44-MAPK
• ERT2
• Extracellular signal-regulated kinase 1
• extracellular signal-related kinase 1
• HS44KDAP
• HUMKER1A
• Insulin-stimulated MAP2 kinase
• MAP kinase 1
• MAP kinase 3
• MAP kinase isoform p44
• MAPK 1
• MAPK 3
• MAPK3
• MGC20180
• Microtubule-associated protein 2 kinase
• Mitogen-activated protein kinase 1
• mitogen-activated protein kinase 3
• P44ERK1
• p44-ERK1
• p44mapk
• PRKM3
• PRKM3EC 2.7.11.24

Background

Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPK's) ERK1 and ERK2 of 44 kDa and 42 kDa, respectively. When activated, ERK's form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the up-regulation of immediate early genes. As such, ERK1 and ERK2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce signals from diverse extracellular stimuli. Alternative splice transcript variants encoding different protein isoforms have been described. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK3
• Uniprot:
  • P27361()
  • P27361()
  • P27361()
  • P27361()
  • P27361()