ERK1/2 Antibody (B20-U)


Western Blot: ERK1/2 Antibody (B20-U) [NBP1-30128] - Left Lane: Marker, Right Lane: Rat brain crude lysate (100ug of protein loaded).

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P, IP
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

ERK1/2 Antibody (B20-U) Summary

Peptide derived from C-terminal sequence of human Erk 2.
Major clone of IgGs representing both, Erk1 (Gene ID: 5595, UniProt: P27361) and Erk2 (Gene ID: 5594, UniProt: P28482), obtained from immunoaffinity purified immunoglobulins corresponding to immunogenic peptide.
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA
0.05% Sodium Azide
Protein A or G purified


  • Western Blot 1:5000
  • ELISA 1:20000-1:100000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
For western blots, incubate the membrane with antibody diluted in blocking buffer 2 hours at room temperature. Western immunoblotting solutions: Wash buffer: 1x Tris Buffered Saline (TBS); 0.2% Triton X-100. Blocking buffer: 1xTBS; 0.2% Triton X-100; 8% nonfat dry milk.

Reactivity Notes

Human, Mouse, and Rat.

Alternate Names for ERK1/2 Antibody (B20-U)

  • EC 2.7.11
  • ERK-1
  • ERK1p44-MAPK
  • ERT2
  • Extracellular signal-regulated kinase 1
  • extracellular signal-related kinase 1
  • HS44KDAP
  • Insulin-stimulated MAP2 kinase
  • MAP kinase 1
  • MAP kinase 3
  • MAP kinase isoform p44
  • MAPK 1
  • MAPK 3
  • MGC20180
  • Microtubule-associated protein 2 kinase
  • Mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 3
  • p44erk1
  • p44-ERK1
  • p44mapk


Erk1 and Erk2 are closely related mitogen activated protein (MAP) kinases which are activated by many growth factors, mitogens and differentiation-promoting agents via a protein kinase cascade. Also known as extracellular signal-regulated kinase 1 and 2, p44/p42 MAP kinases, microtubule-associated protein-2 kinases, myelin basic protein (MBP) kinases or EGF receptor T669 (Ert) kinases (2-5), Erk1 and Erk2 are ubiquitous and abundant, although their relative abundance in specific tissues may vary. The two kinases are nearly 85% identical and have higher identity in the core regions involved in substrate binding 5. Erk1 and Erk2 are activated approximately 1000-fold by phosphorylation within a Thr-Glu-Tyr motif in the activation loop on both threonine and tyrosine residues by Mek1 and Mek2 (5,6). Both sites must be phosphorylated for maximum activity (2-5). These kinases in turn phosphorylate a variety of different substrates. Erk1 and Erk2 are found in the cytoplasm and are translocated to the nucleus upon activation. Erk1 and Erk2 target membrane proteins, cytosolic proteins, such as downstream kinases, and cytoskeletal proteins and nuclear proteins, such as transcription factors. Many of these substrates are important regulatory proteins. Erk1 and Erk2 represent proximal kinases in the classical kinase pathway which links growth and differentiation signals at the cell surface (through tyrosine kinases) with transcription in the nucleus.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
Species: Hu
Applications: WB, PEP-ELISA
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Ce, Hu(-), Ma(-), Mu(-), Rt(-)
Applications: WB, ELISA, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC
Species: Hu
Applications: WB, Flow, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, PEP-ELISA
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
Species: Hu, Mu, Rt, Po, Ca
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for ERK1/2 Antibody (NBP1-30128) (0)

There are no publications for ERK1/2 Antibody (NBP1-30128).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ERK1/2 Antibody (NBP1-30128) (0)

There are no reviews for ERK1/2 Antibody (NBP1-30128). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ERK1/2 Antibody (NBP1-30128) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional ERK1/2 Antibody (B20-U) Products

Related Products by Gene

Bioinformatics Tool for ERK1/2 Antibody (NBP1-30128)

Discover related pathways, diseases and genes to ERK1/2 Antibody (NBP1-30128). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ERK1/2 Antibody (NBP1-30128)

Discover more about diseases related to ERK1/2 Antibody (NBP1-30128).

Pathways for ERK1/2 Antibody (NBP1-30128)

View related products by pathway.

PTMs for ERK1/2 Antibody (NBP1-30128)

Learn more about PTMs related to ERK1/2 Antibody (NBP1-30128).

Research Areas for ERK1/2 Antibody (NBP1-30128)

Find related products by research area.

Blogs on ERK1/2.

SUCNR1/GPR91 - a potential role in renovascular hypertension
SUCNR1 is the cognate receptor for the Kreb's citric acid cycle intermediate succinate. It is of interest to scientists because it is involved in not only energy metabolism but possibly also in renovascular hypertension, a condition linked to diabe...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol MAPK3

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-30128 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought