ERG Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ERG Antibody [NBP1-31370] - Analysis of HeLa, using NBP1-31370 at 1:200 dilution.

Western Blot: ERG Antibody [NBP1-31370] - 30 ug Raw264. 7 whole lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERG antibody (NBP1-31370) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ERG Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ERG Antibody - BSA Free (NBP1-31370) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-ERG Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human ERG. The exact sequence is proprietary.
• Localization: Nucleus |
• Predicted Species: Rat (97%), Porcine (93%), Bovine (95%), Chicken (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ERG
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Paraffin Reported in scientific literature (PMID:35124460)
  • Western Blot 1:500-1:3000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ERG Antibody - BSA Free

• transcriptional regulator ERG
• 10ets-related
• Erg
• Erg-3
• p55
• TMPRSS2-ERG prostate cancer specific
• transcriptional regulator ERG
• Transforming protein ERG
• v-ets erythroblastosis virus E26 oncogene homolog (avian)
• v-ets erythroblastosis virus E26 oncogene like (avian)
• v-ets erythroblastosis virus E26 oncogene like

Background

Gene ID(s): 2078 (human)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ERG Antibody (NBP1-31370)(1)

Publication using NBP1-31370

• Takemura S, Ohta Y, Sugama R et al. Eldecalcitol regulates the gene expressions of articular cartilage markers and differentiation markers in chondrocytes Biochemical and Biophysical Research Communications 2022-02-01 [PMID: 35124460] (IHC-P, Mouse)

FAQs for ERG Antibody (NBP1-31370). (Showing 1 - 1 of 1 FAQ).

1. I am looking for an antibody that can IP Erg protein and doesn't cross react with Fli protein.
   • I have spoken to the lab and they have informed me that actually that the antibody (catalog #NBP1-95857) is confirmed not to cross react with Fli1 protein so it should be a viable choice for you in your experiments on Erg.

Bioinformatics

• Gene Symbol: ERG