ErbB2/Her2 Antibody [Biotin]

ErbB2/Her2 was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human ErbB2/Her2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1129) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human ErbB2/Her2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1129) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, ELISA(Det), ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

ErbB2/Her2 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ErbB2
  Thr23-Thr652
  Accession # P04626
• Specificity: Detects human ErbB2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human (rh) ErbB3, rhErbB4, and rhEGF R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ERBB2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human ErbB2/Her2 Antibody (Catalog # MAB1129)
  ELISA Detection: Human ErbB2/Her2 Biotinylated Antibody (Catalog # BAF1129)
  Standard: Recombinant Human ErbB2/Her2 Fc Chimera (Catalog # 1129-ER)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ErbB2/Her2 Antibody [Biotin]

• CD340 antigen
• CD340
• c-erb B2/neu protein
• EC 2.7.10
• EGFR2
• ErbB2
• HER2
• HER-2
• HER2EC 2.7.10.1
• herstatin
• Metastatic lymph node gene 19 protein
• MLN 19
• MLN19
• Neu Oncogene
• NEUHER-2/neu
• neuroblastoma/glioblastoma derived oncogene homolog
• NGL
• NGLTKR1
• p185erbB2
• Proto-oncogene c-ErbB-2
• Proto-oncogene Neu
• receptor tyrosine-protein kinase erbB-2
• TKR1
• Tyrosine kinase-type cell surface receptor HER2
• v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
• v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Background

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGF R), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1-10).

1. Coussens, L. et al. (1985) Science 230:1132.
2. Yamamoto, T. et al. (1986) Nature 319:230.
3. Kanai, Y. et al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
4. Codony-Servat, J. et al. (1999) Cancer Res. 59:1196.
5. Carraway, K.L. 3^rd et al.  (1994) J. Biol. Chem. 269:14303.
6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
7. Schaefer, G. et al. (1999) J. Biol. Chem. 274:859.
8. Schlessinger, J. (2000) Cell 103:211.
9. Hellyer, N.J. et al. (2001) J. Biol. Chem. 276:42153.
10. Daly, R.J. (1999) Growth Factors 16:255.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ErbB2/Her2 Antibody (BAF1129)(14)

Publications using BAF1129

• Boonkaew, S;Teodori, L;Vendelbo, MH;Kjems, J;Ferapontova, EE; Nanobodies' duo facilitates ultrasensitive serum HER-2/neu immunoassays via enhanced avidity interactions Analytica chimica acta 2025-01-15 [PMID: 39643321] (Immunoassay Development, ELISA Detection, Human, N/A)
• Ana Díaz-Fernández, Alexey Ferapontov, Mikkel Holm Vendelbo, Elena E. Ferapontova Electrochemical Cellulase-Linked ELASA for Rapid Liquid Biopsy Testing of Serum HER-2/neu ACS Measurement Science Au 2023-06-21 [PMID: 37360033]
• A Díaz-Ferná, EE Ferapontov Covalent Hemin/G4 complex-linked sandwich bioassay on magnetic beads for femtomolar HER-2/neu detection in human serum via direct electrocatalytic reduction of oxygen Oncogene, 2022-06-06;1219(0):340049. 2022-06-06 [PMID: 35715131] (Functional Assay, N/A)
• J Liu, Y Qu, H Wang Immuno-Acoustic Sorting of Disease-Specific Extracellular Vesicles by Acoustophoretic Force Micromachines, 2021-12-09;12(12):. 2021-12-09 [PMID: 34945384] (ELISA Capture, Human)
• Luca Gerosa, Christopher Chidley, Fabian Fröhlich, Gabriela Sanchez, Sang Kyun Lim, Jeremy Muhlich et al. Receptor-Driven ERK Pulses Reconfigure MAPK Signaling and Enable Persistence of Drug-Adapted BRAF-Mutant Melanoma Cells Cell Systems 2020-11-01 [PMID: 33113355]
• V Agnolon, A Contato, A Meneghello, E Tagliabue, G Toffoli, M Gion, F Polo, ASC Fabricio ELISA assay employing epitope-specific monoclonal antibodies to quantify circulating HER2 with potential application in monitoring cancer�patients undergoing therapy with trastuzumab Sci Rep, 2020-02-20;10(1):3016. 2/20/2020 [PMID: 32080226]
• K Malecka, D Pankratov, EE Ferapontov Femtomolar electroanalysis of a breast cancer biomarker HER-2/neu protein in human serum by the cellulase-linked sandwich assay on magnetic beads Anal. Chim. Acta, 2019-05-25;1077(0):140-149. 2019-05-25 [PMID: 31307703] (ELISA Capture, ELISA Detection, Human)
• Allison M. Claas, Lyla Atta, Simon Gordonov, Aaron S. Meyer, Douglas A. Lauffenburger Systems Modeling Identifies Divergent Receptor Tyrosine Kinase Reprogramming to MAPK Pathway Inhibition Cellular and Molecular Bioengineering 2018-12-01 [PMID: 30524510]
• Marine De Paoli, Selma Gogalic, Ursula Sauer, Claudia Preininger, Hardev Pandha, Guy Simpson et al. Multiplatform Biomarker Discovery for Bladder Cancer Recurrence Diagnosis Disease Markers 2016-08-31 [PMID: 27660385]
• Sarah Javaid, Jianmin Zhang, Gromoslaw A. Smolen, Min Yu, Ben S. Wittner, Anurag Singh et al. MAPK7 Regulates EMT Features and Modulates the Generation of CTCs Molecular Cancer Research 2015-05-01 [PMID: 25678598]
• Shiddiky , Muhammad, Vaidyanathan , Ramanath, Rauf , Sakandar, Tay , Zhikai, Trau , Matt Molecular nanoshearing: an innovative approach to shear off molecules with AC-induced nanoscopic fluid flow. Sci Rep, 2014-01-16;4(0):3716. 2014-01-16 [PMID: 24430114] (Functional Assay, Human)
• Jin-Hee Han, L. Sudheendra, Hee-Joo Kim, Shirley J. Gee, Bruce D. Hammock, Ian M. Kennedy Ultrasensitive On-Chip Immunoassays with a Nanoparticle-Assembled Photonic Crystal ACS Nano 2012-10-23 [PMID: 22957818]
• Khan IH, Zhao J, Ghosh P, Ziman M, Sweeney C, Kung HJ, Luciw PA Microbead arrays for the analysis of ErbB receptor tyrosine kinase activation and dimerization in breast cancer cells. Assay Drug Dev Technol, 2010-02-01;8(1):27-36. 2010-02-01 [PMID: 20035613] (Luminex Development, Western Blot, Human)
• Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human)

FAQs for ErbB2/Her2 Antibody (BAF1129). (Showing 1 - 1 of 1 FAQs).

1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
   • A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

Bioinformatics

• Gene Symbol: ERBB2
• Uniprot:
  • P04626()