ER alpha/NR3A1 Antibody (6B6)

Western Blot: ER alpha/NR3A1 Antibody (6B6) [NBP1-51653] - Western blot analysis using ESR1 mouse mAb against MCF-7 (1), T47D (2) and SKBR3 (3) cell lysate.

Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6B6) [NBP1-51653] - Immunofluorescence analysis of Hela cells using ESR1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6B6) [NBP1-51653] - Immunohistochemical analysis of paraffin-embedded mammary cancer tissues using ESR1 mouse mAb with DAB staining.

Product Discontinued

ER alpha/NR3A1 Antibody (6B6) Summary

• Immunogen: Purified recombinant fragment of human ESR1 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ESR1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for ER alpha/NR3A1 Antibody (6B6)

• ER alpha
• ER
• Era
• ER-alpha
• ESR1
• ESRESRA
• Estradiol receptor
• estrogen receptor 1
• estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
• estrogen receptor alpha delta 4 +49 isoform
• estrogen receptor alpha delta 4*5,6,7*/654 isoform
• estrogen receptor alpha
• estrogen receptor
• NR3A1
• NR3A1DKFZp686N23123
• Nuclear receptor subfamily 3 group A member 1

Background

ERalpha (Estrogen receptor alpha) belongs to the nuclear hormone receptor family, NR3 subfamily. Contains an N-terminal domain with a hormone-independent transcriptional activation function (AF-1), a central DNA-binding domain, and a C-terminal ligand-binding domain with a hormone-dependent transcriptional activation function (AF-2). Predicted molecular weight approximately 66 kD. Estrogen receptor has two subtypes, ERalpha and ERbeta. Liganded ERalpha and ERbeta binds as a homodimer or as a hetrodimer with an estrogen responsive element and regulate the target gene transcription. Estrogen receptors mediate most of the biological effects of estrogen in mammary and uterine epithelial cells. High expression of ERs is observed in a large population of breast tumors. It has been reported AKT1, AKT2, p90RSK3, or p90RSK1 are capable to phosphorylate ser-167 of the human ER and Ser-167 may play a role in controlling AF-1 function. The Poly6365 antibody recognizes human phospho-ERalpha (Ser 167) and has been shown to be useful for immunoflorence staining. This antibody does not detect ERalpha phosphorylated at other sites. BRCA1, BRN3A, CDC 25B, Calmodulin 1-3, Cyclin H, ERK1, SUG1, NRIF3, Cyclin D1, Grb2, DDX5, IKKgamma, TIF2, PAK1, NCOA3, p90RSK1, ERbeta, STAT5A, ERK2, SHP1, c-Jun, MDM2, Lck, Tuberin, SP1, SP3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESR1