ER alpha/NR3A1 Antibody (585112)

ERa/NR3A1 was detected in immersion fixed PC-3 human prostate cancer cell line using Mouse Anti-Human ERa/NR3A1 Monoclonal Antibody (Catalog # MAB5715) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counter-stained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 585112
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ER alpha/NR3A1 Antibody (585112) Summary

• Immunogen: E. coli-derived recombinant human ER alpha /NR3A1
  Met1-Ser116 (predicted)
  Accession # P03372
• Specificity: Detects human ER alpha /NR3A1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant ER beta /NR3A2 is observed.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: ESR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ER alpha/NR3A1 Antibody (585112)

• ER alpha
• ER
• Era
• ER-alpha
• ESR1
• ESRESRA
• Estradiol receptor
• estrogen receptor 1
• estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
• estrogen receptor alpha delta 4 +49 isoform
• estrogen receptor alpha delta 4*5,6,7*/654 isoform
• estrogen receptor alpha
• estrogen receptor
• NR3A1
• NR3A1DKFZp686N23123
• Nuclear receptor subfamily 3 group A member 1

Background

ER alpha (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65-70 kDa member of the NR3 subfamily, nuclear hormone receptor family of proteins. It is widely expressed, and serves as a strong activator of estrogen-responsive genes. ER alpha is normally quiescent and bound to heat-shock proteins and immunophilins. Following beta -estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ER beta , and binds to DNA with multiple coactivators. Human ER alpha is 595 amino acids (aa) in length.  It contains a DNA binding region (aa 185-250), three NLSs (aa 256-260; 266-271; 299-303), a steroid-binding site (aa 351-543), a dimerization motif (aa 497-518), and an O-GlcNAc attachment around Thr575. Major phosphorylation sites exist at Tyr537, Ser167 and Ser118. Multiple splice forms exist. There is an 80 kDa isoform that shows a substitution (duplication) of aa 412-517 for Asp411, a second isoform with a deletion of aa 255-366, a third isoform with a deletion of aa 152-412, and a fourth isoform that shows a Thr substitution for aa 152-595. Human ER alpha is only 46% aa identical to human ER beta . Over aa 1-116, human ER alpha shares 85% aa identity with mouse ER alpha .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESR1
• Uniprot:
  • P03372()