ER alpha/NR3A1 Antibody (585112) [Alexa Fluor® 488]

• Applications: ICC/IF
• Clone: 585112
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

ER alpha/NR3A1 Antibody (585112) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human ERα/NR3A1
  Met1-Ser116 (predicted)
  Accession # P03372
• Specificity: Detects human ER alpha /NR3A1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant ER beta /NR3A2 is observed.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: ESR1
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

ER alpha (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65-70 kDa member of the NR3 subfamily, nuclear hormone receptor family of proteins. It is widely expressed, and serves as a strong activator of estrogen-responsive genes. ER alpha is normally quiescent and bound to heat-shock proteins and immunophilins. Following beta -estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ER beta , and binds to DNA with multiple coactivators. Human ER alpha is 595 amino acids (aa) in length.  It contains a DNA binding region (aa 185-250), three NLSs (aa 256-260; 266-271; 299-303), a steroid-binding site (aa 351-543), a dimerization motif (aa 497-518), and an O-GlcNAc attachment around Thr575. Major phosphorylation sites exist at Tyr537, Ser167 and Ser118. Multiple splice forms exist. There is an 80 kDa isoform that shows a substitution (duplication) of aa 412-517 for Asp411, a second isoform with a deletion of aa 255-366, a third isoform with a deletion of aa 152-412, and a fourth isoform that shows a Thr substitution for aa 152-595. Human ER alpha is only 46% aa identical to human ER beta . Over aa 1-116, human ER alpha shares 85% aa identity with mouse ER alpha .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESR1