Ephrin-B1 [p Tyr317] Antibody - Azide Free

Western Blot: Ephrin-B1 [p Tyr317] Antibody [NB300-275] - Rat testes lysate showing specific immunolabeling of the ~46k EphriNBphosphorylated at Tyr317 (control, lane 1). The phosphospecificity of this labeling is shown in lane 2 (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the Anti-Tyr317 EphrinB. The immunolabeling of the EphriNBis completely eliminated by treatment with lambda-phosphatase.

• Reactivity: Mu, Rt, Hu, Bv, Ch, Xp, Ze
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Ephrin-B1 [p Tyr317] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ephrin-B1 conjugated to KLH. Accession # P28693
• Modification: p Tyr317
• Specificity: Specific for endogenous levels of the ~46 kDa Ephrin-B1 protein phosphorylated at Tyr317. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Predicted Species: Human (100%), Chicken (100%), Xenopus (100%), Bovine (100%), Zebrafish (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EFNB1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:1000
• Application Notes: This antibody has been tested in Western blot on rat lysate, detecting a band ~46 kDa. Immunofluorescence (PMID: 21530605)
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Tested in Rat. Mouse reactivity reported in scientific literature (PMID: 21539827).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Ephrin-B1 [p Tyr317] Antibody - Azide Free

• Cek5-L
• EFL3
• EFL-3
• EFNB1
• ELK ligand
• ELK-L
• EphrinB1
• Ephrin-B1
• LERK-2
• STRA-1

Background

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event. Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling. (3, 5)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ephrin-B1 Antibody (NB300-275)(1)

Publication using NB300-275

• Dravis C, Henkemeyer M. Ephrin-B reverse signaling controls septation events at the embryonic midline through separate tyrosine phosphorylation-independent signaling avenues Dev Biol 2011-07-01 [PMID: 21539827] (WB, ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: EFNB1