Ephrin-A4 Antibody [Unconjugated]

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Ephrin‑A4 was detected in immersion fixed paraffin-embedded sections of normal human mammary glands using Goat Anti-Human Ephrin‑A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF369) at 3 µg/mL ...read more
Ephrin‑A4 was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human Ephrin‑A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF369) at 5 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Ephrin-A4 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Ephrin-A4
Leu26-Gly171
Accession # P52798
Specificity
Detects human Ephrin-A4 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse Ephrin-A4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EFNA4
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF369 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ephrin-A4 Antibody [Unconjugated]

  • EFL4
  • EFL-4
  • EFNA4
  • EPH-related receptor tyrosine kinase ligand 4
  • EphrinA4
  • Ephrin-A4
  • EPLG4MGC125826
  • LERK-4
  • LERK4FLJ57652
  • ligand of eph-related kinase 4

Background

Ephrin-A4, also known as LERK-4 and EFL-4, (1) is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A4 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 (2, 3). The extracellular domains of human and mouse Ephrin-A4 share 80% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ephrin-A4 Antibody (AF369)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Immunoprecipitation, Western Blot.


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Immunoprecipitation
(1)
Western Blot
(1)
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(2)
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Bioinformatics

Gene Symbol EFNA4
Uniprot