Ephrin-A4 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Ephrin-A4 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Ephrin-A4
  Leu26-Gly171
  Accession # P52798
• Specificity: Detects human Ephrin-A4 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse Ephrin-A4 is observed, 5% cross-reactivity with recombinant human (rh) Ephrin-A5 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) Ephrin-A2, rhEphrin-A3, rmEphrin-A1, rmEphrin-B1, and rmEphrin-B2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EFNA4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ephrin-A4 Antibody [Biotin]

• EFL4
• EFL-4
• EFNA4
• EPH-related receptor tyrosine kinase ligand 4
• EphrinA4
• Ephrin-A4
• EPLG4MGC125826
• LERK-4
• LERK4FLJ57652
• ligand of eph-related kinase 4

Background

Ephrin-A4, also known as LERK-4 and EFL-4, (1) is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A4 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 (2, 3). The extracellular domains of human and mouse Ephrin-A4 share 80% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ephrin-A4 Antibody (BAF369)(1)

Publication using BAF369

• Finne EF, Munthe E, Aasheim HC A new ephrin-A1 isoform (ephrin-A1b) with altered receptor binding properties abrogates the cleavage of ephrin-A1a. Biochem. J., 2004-04-01;379(0):39-46. 2004-04-01 [PMID: 14692877] (Flow Cytometry, Western Blot, Human)

Bioinformatics

• Gene Symbol: EFNA4
• Uniprot:
  • P52798()