Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free

Western Blot: Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free [NBP2-70622] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ephrin A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ephrin A2.

Immunocytochemistry/Immunofluorescence: Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free [NBP2-70622] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Ephrin A2.

Immunohistochemistry: Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free [NBP2-70622] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Ephrin A2 mouse monoclonal antibody.

Flow Cytometry: Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free [NBP2-70622] - Analysis of Jurkat cells, using anti-Ephrin A2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI3E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free (NBP2-02810) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human EFNA2 (NP_001396) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: EFNA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 21.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free

• Cek7-L
• EFNA2
• ELF-1
• EPH-related receptor tyrosine kinase ligand 6
• EphrinA2
• Ephrin-A2
• EPLG6HEK7 ligand
• HEK7-L
• HEK7-ligand
• LERK6
• LERK6LERK-6
• ligand of eph-related kinase 6

Background

The Eph subfamily represents the largest group of receptor protein kinases identified to date. There is increasing evidence that Eph family members are involved in central nervous system function and in development. Ligands for Eph receptors include ephrin-A1 (LERK-1/B61), identified as a ligand for the EphA2 (Eck) receptor; ephrin-A2 (ELF-1), identified as a ligand for the EphA3 and EphA4 (Sek) receptors; ephrin-A3 (LERK-3), identified as a ligand for EphA5 (Ehk1) and EphA3 (Hek) receptors; ephrin-A4 (LERK-4), identified as a ligand for the EphA3 receptor; ephrin-A5 (AL-1), identified as a ligand for EphA5 (REK7); ephrin-B1 (LERK-2), identified as a ligand for the EphB1 (Elk) and EphB2 (Cek5) receptors; ephrin-B2 (LERK-5), identified as a ligand for the EphB1, EphB3 (Cek10) and EphB2 receptors; and ephrin-B3 (LERK-8), identified as a ligand for EphB1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EFNA2