Ephrin-A2 Antibody (OTI3E3)

Western Blot: Ephrin A2 Antibody (3E3) [NBP2-02810] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ephrin A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ephrin A2.

Immunocytochemistry/Immunofluorescence: Ephrin A2 Antibody (3E3) [NBP2-02810] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Ephrin A2.

Immunohistochemistry-Paraffin: Ephrin A2 Antibody (3E3) [NBP2-02810] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Ephrin A2 mouse monoclonal antibody.

Flow Cytometry: Ephrin A2 Antibody (3E3) [NBP2-02810] - Analysis of Jurkat cells, using anti-Ephrin A2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI3E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Ephrin-A2 Antibody (OTI3E3) Summary

• Immunogen: Full length human recombinant protein of human EFNA2 (NP_001396) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: EFNA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 21.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ephrin-A2 Antibody (OTI3E3)

• Cek7-L
• EFNA2
• ELF-1
• EPH-related receptor tyrosine kinase ligand 6
• EphrinA2
• Ephrin-A2
• EPLG6HEK7 ligand
• HEK7-L
• HEK7-ligand
• LERK6
• LERK6LERK-6
• ligand of eph-related kinase 6

Background

This gene encodes a member of the ephrin family. The protein is composed of a signal sequence, a receptor-binding region, a spacer region, and a hydrophobic region. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Posttranslational modifications determine whether this protein localizes to the nucleus or the cytoplasm. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ephrin-A2 Antibody (NBP2-02810)(1)

Publication using NBP2-02810

• Taketo Ezaki, Toshihide Tanaka, Ryota Tamura, Kentaro Ohara, Yohei Yamamoto, Jun Takei, Yukina Morimoto, Ryotaro Imai, Yuki Kuranai, Yasuharu Akasaki, Masahiro Toda, Yuichi Murayama, Keisuke Miyake, Hikaru Sasaki Status of alternative angiogenic pathways in glioblastoma resected under and after bevacizumab treatment Brain Tumor Pathology 2024-04-15 [PMID: 38619734]

Bioinformatics

• Gene Symbol: EFNA2
• Entrez:
  • 1943(Human)