EphB4 Antibody (395810) [Phycoerythrin]

Images

 
MCF‑7 human breast cancer cell line was stained with Rat Anti-Human EphB4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3038P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
395810
Clonality
Monoclonal
Host
Rat
Conjugate
Phycoerythrin

EphB4 Antibody (395810) [Phycoerythrin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human EphB4
Ala16-Ala539
Accession # AAH52804
Specificity
Detects human EphB4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human EphA1, A2, A5, A6, A10, B2, B3, B6, recombinant mouse EphA3, A4, or recombinant rat EphB1 is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
EPHB4
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Purity
Protein A or G purified from hybridoma culture supernatant

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphB4 Antibody (395810) [Phycoerythrin]

  • EC 2.7.10
  • EC 2.7.10.1
  • EPH receptor B4
  • EphB4
  • ephrin type-B receptor 4
  • hepatoma transmembrane kinase
  • Htk
  • HTKephrin receptor EphB4
  • Mdk2
  • Myk1
  • soluble EPHB4 variant 1
  • soluble EPHB4 variant 2
  • soluble EPHB4 variant 3
  • Tyro11
  • Tyrosine-protein kinase receptor HTK
  • Tyrosine-protein kinase TYRO11

Background

EphB4, also known as Htk, Myk1, Tyro11, and Mdk2, is a member of the Eph receptor tyrosine kinase family and binds Ephrin-B2. The A and B class Eph proteins have a common structural organization (1‑4). The human EphB4 cDNA encodes a 987 amino acid precursor that includes a 15 amino acid (aa) signal sequence, a 524 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 427 aa cytoplasmic domain (5). The ECD contains an N-terminal globular domain, a cysteine‑rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved Sterile Alpha Motif (SAM) (5). Activation of kinase activity occurs after membrane-bound or clustered ligand recognition and binding. The ECD of human EphB4 shares 89% aa sequence identity with mouse EphB4 and 42‑45% aa sequence identity with human EphB1, 2, and 3. EphB4 is expressed preferentially on venous Endothelial Cells (EC) and inhibits cell-cell adhesion, chemotaxis, and angiogenesis. Opposing effects are induced by signaling through Ephrin-B2 expressed on arterial EC: adhesion, endothelial cell migration, and vessel sprouting (6). EphB4 signaling contributes to new vascularization by guiding venous EC away from Ephrin-B2 expressing EC. Ephrin-B2 signaling induces arterial EC to migrate towards nascent EphB4 expressing vessels (6). The combination of forward signaling through EphB4 and reverse signaling through Ephrin-B2 promotes in vivo mammary tumor growth and tumor‑associated angiogenesis (7). EphB4 promotes the differentiation of megakaryocytic and erythroid progenitors but not granulocytic or monocytic progenitors (8, 9).

  1. Poliakov, A. et al. (2004) Dev. Cell 7:465.
  2. Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419.
  3. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  4. Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
  5. Bennett, B.D. et al. (1994) J. Biol. Chem. 269:14211.
  6. Fuller, T. et al. (2003) J. Cell Sci. 116:2461.
  7. Noren, N.K. et al. (2004) Proc. Natl. Acad. Sci. 101:5583.
  8. Wang, Z. et al. (2002) Blood 99:2740.
  9. Inada, T. et al. (1997) Blood 89:2757.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB3038U
Alexa Fluor 405 FAB3038V
Alexa Fluor 488 FAB3038G
Alexa Fluor 594 FAB3038T
Alexa Fluor 647 FAB3038R
Alexa Fluor 700 FAB3038N
Alexa Fluor 750 FAB3038S
Phycoerythrin FAB3038P

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Bioinformatics

Gene Symbol EPHB4
Uniprot
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