EphA4 [p Tyr602] Antibody

Western Blot: EphA4 [p Tyr602] Antibody [NBP3-23329] - Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

EphA4 [p Tyr602] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: EphA4 (Tyr-602) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding phosphorylated tyrosine 602 in human EphA4. This sequence has significant homology to the conserved site in rat and mouse EphA4, and is conserved well in most other EphA and EphB family members. [UniProt# P54764]
• Modification: p Tyr602
• Specificity: This antibody was cross-adsorbed to unrelated phosphotyrosine peptides before affinity purification using EphA4 (Tyr-602) peptide. The purified antibody detects a 120 kDa band corresponding to EphA4 in Western blots of HUVEC cells treated with pervanadate and in mouse brain tissue.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EPHA4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100
  • Western Blot 1:1000
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for EphA4 [p Tyr602] Antibody

• Cek8
• EC 2.7.10
• EC 2.7.10.1
• EK8
• EPH receptor A4
• EphA4
• EPH-like kinase 8
• ephrin type-A receptor 4
• Hek8
• receptor protein-tyrosine kinase HEK8
• SEK
• Sek1
• TYRO1 protein tyrosine kinase
• Tyro1
• Tyrosine-protein kinase receptor SEK
• Tyrosine-protein kinase TYRO1

Background

Eph receptor A4 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPHA4