EphA3 Antibody [Biotin]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

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EphA3 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphA3
Met1-His541 (Thr323Ala, Glu475Gln)
Accession # Q8BRB1
Specificity
Detects mouse EphA3 in Western blots. In Western blots (under non-reducing conditions), approximately 5% cross‑reactivity with recombinant mouse (rm) EphA7, recombinant rat (rr) EphA5, rmEphA2, rmEphA4, rmEphA8, and rmEphA6 is observed and less than 1% cross-reactivity with rrEphB1, rmEphB2, and rmEphB3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EPHA3
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Applications/Dilutions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphA3 Antibody [Biotin]

  • Cek4
  • EC 2.7.10
  • EC 2.7.10.1
  • EK4
  • EPH receptor A3
  • EphA3
  • EPH-like kinase 4
  • ephrin type-A receptor 3
  • ETK
  • Hek4
  • HEKETK1eph-like tyrosine kinase 1
  • human embryo kinase 1
  • TYRO4 protein tyrosine kinase
  • Tyro4
  • Tyrosine-protein kinase receptor ETK1
  • Tyrosine-protein kinase TYRO4

Background

EphA3, also known as Cek4, Mek4, Hek, Tyro4, and Hek4 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA3 has been shown to bind ephrin-A5, ephrin-A2, ephrin-A3, ephrin-A1, ephrin-A4, and ephrin-B1 (2, 3). The extracellular domains of mouse and human EphA3 share greater than 96% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EPHA3
Entrez
Uniprot