CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="EphA3 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse EphA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF640) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF640-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB640-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-17708-25ul | |||
![Western Blot: EphA3 Antibody [NBP2-16346] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/EphA3-Antibody-Western-Blot-NBP2-16346-img0008.jpg "Western Blot: EphA3 Antibody [NBP2-16346] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunohistochemistry-Paraffin: EphA3 Antibody [NBP2-16346] - Human gastric cancer, using Eph Receptor A3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/EPH-Receptor-A3-Antibody-Immunohistochemistry-Paraffin-NBP2-16346-img0002.jpg)
![Western Blot: EphA3 Antibody [NBP2-16346] - Mouse tissue extract (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EphA3-Antibody-Western-Blot-NBP2-16346-img0004.jpg)
![Western Blot: EphA3 Antibody [NBP2-16346] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EphA3-Antibody-Western-Blot-NBP2-16346-img0006.jpg)
![Western Blot: EphA3 Antibody [NBP2-16346] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] (NBP2-16346) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16346_rabbit-polyclonal-epha3-antibody-910202420134076.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] (NBP2-16346) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16346_rabbit-polyclonal-epha3-antibody-western-blot-2611202518393115.jpg)
![Western Blot: EphA3 Antibody (3A12) [H00002042-M01] - EPHA3 monoclonal antibody (M01), clone 3A12. Analysis of EPHA3 expression in human spleen.](http://images.novusbio.com/fullsize/EphA3-Antibody-3A12-Western-Blot-H00002042-M01-img0006.jpg "Western Blot: EphA3 Antibody (3A12) [H00002042-M01] - EPHA3 monoclonal antibody (M01), clone 3A12. Analysis of EPHA3 expression in human spleen.")
![Western Blot: EphA3 Antibody (3A12) [H00002042-M01] - analysis of EPHA3 expression in transfected 293T cell line by EPHA3 monoclonal antibody (M01), clone 3A12. Lane 1: EPHA3 transfected lysate (110.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/EphA3-Antibody-3A12-Western-Blot-H00002042-M01-img0001.jpg)
![Western Blot: EphA3 Antibody (3A12) [H00002042-M01] - Analysis of EPHA3 over-expressed 293 cell line, cotransfected with EPHA3 Validated Chimera RNAi ( Cat # H00002042-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EPHA3 monoclonal antibody (M01) clone 3A12 (Cat # H00002042-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/EphA3-Antibody-3A12-Western-Blot-H00002042-M01-img0004.jpg)
![Sandwich ELISA: EphA3 Antibody (3A12) [H00002042-M01] - Detection limit for recombinant GST tagged EPHA3 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/EphA3-Antibody-3A12-Sandwich-ELISA-H00002042-M01-img0005.jpg)
![Western Blot: EphA3 Antibody [NBP3-12465] - SKMNC cells. 1:500 antibody dilution in DiluObuffer. Apparent MW is 110 KDa.](http://images.novusbio.com/fullsize/EphA3-Antibody-Western-Blot-NBP3-12465-img0001.jpg "Western Blot: EphA3 Antibody [NBP3-12465] - SKMNC cells. 1:500 antibody dilution in DiluObuffer. Apparent MW is 110 KDa.")
![Western Blot: EphA3 Antibody (3E9) [H00002042-M02] - EPHA3 monoclonal antibody (M02), clone 3E9 Analysis of EPHA3 expression in HeLa.](http://images.novusbio.com/fullsize/EphA3-Antibody-3E9-Western-Blot-H00002042-M02-img0004.jpg "Western Blot: EphA3 Antibody (3E9) [H00002042-M02] - EPHA3 monoclonal antibody (M02), clone 3E9 Analysis of EPHA3 expression in HeLa.")
![Sandwich ELISA: EphA3 Antibody (3E9) [H00002042-M02] - Detection limit for recombinant GST tagged EPHA3 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/EphA3-Antibody-3E9-Sandwich-ELISA-H00002042-M02-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: EphA3 Antibody [NBP3-17708] - Staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane, cytosol & actin filaments.](http://images.novusbio.com/fullsize/EphA3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17708-img0001.jpg "Immunocytochemistry/Immunofluorescence: EphA3 Antibody [NBP3-17708] - Staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane, cytosol & actin filaments.")
![Western Blot: EPH Receptor A3 Overexpression Lysate (Adult Normal) [NBP2-10482] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EPH Receptor A3.](http://images.novusbio.com/fullsize/EPH-Receptor-A3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10482-img0001.jpg "Western Blot: EPH Receptor A3 Overexpression Lysate (Adult Normal) [NBP2-10482] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EPH Receptor A3.")
![ELISA: Human EphA3 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31536] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31536_human-epha3-ready-to-use-elisa-kit-colorimetric-206202415314852.png "ELISA: Human EphA3 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31536] - Standard Curve Reference")
