EphA2 Antibody (M088)

ELISA: EphA2 Antibody (M088) [NBP3-23321] - Representative Standard Curve using mouse monoclonal anti-EphA2 (NBP3-23321) for ELISA capture of human recombinant EphA2 extracellular region with a His-tag. Captured protein was detected by suitable anti-His-tag antibody followed by appropriate secondary antibody HRP conjugate.

Immunocytochemistry/Immunofluorescence: EphA2 Antibody (M088) [NBP3-23321] - Immunocytochemical labeling of EphA2 in aldehyde fixed human MDA-MB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-EphA2 (NBP3-23321). The antibody was detected using goat anti-mouse DyLight(R) 594.

• Reactivity: Hu
• Applications: ICC/IF, IP
• Clone: M088
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

EphA2 Antibody (M088) Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Clone (M088) was generated from a recombinant protein that included the extracellular region of human EphA2 protein. [UniProt# P29317]
• Specificity: Clone M088 mouse monoclonal antibody detects native human EphA2, as well as aldehyde-fixed EphA2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPHA2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunoprecipitation 1:100
• Theoretical MW: 125 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for EphA2 Antibody (M088)

• ARCC2
• EC 2.7.10
• EC 2.7.10.1
• Eck
• ECKepithelial cell receptor protein tyrosine kinase
• EPH receptor A2
• EphA2
• ephrin type-A receptor 2
• Epithelial cell kinase
• Myk2
• Sek2
• soluble EPHA2 variant 1
• Tyrosine-protein kinase receptor ECK

Background

Eph receptor A2 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPHA2