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EphA2 Antibody (1C1) [Janelia Fluor® 549] - Chimeric

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
1C1
Clonality
Monoclonal
Host
Rabbit
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-52677AF350
Alexa Fluor 405
NBP2-52677AF405
Alexa Fluor 488
NBP2-52677AF488
Alexa Fluor 532
NBP2-52677AF532
Alexa Fluor 594
NBP2-52677AF594
Alexa Fluor 647
NBP2-52677AF647
Alexa Fluor 700
NBP2-52677AF700
Alexa Fluor 750
NBP2-52677AF750
Allophycocyanin
NBP2-52677APC
Allophycocyanin/Cy7
NBP2-52677APCCY7
Biotin
NBP2-52677B
DyLight 350
NBP2-52677UV
DyLight 405
NBP2-52677V
DyLight 488
NBP2-52677G
DyLight 550
NBP2-52677R
DyLight 650
NBP2-52677C
DyLight 680
NBP2-52677FR
DyLight 755
NBP2-52677IR
FITC
NBP2-52677F
HRP
NBP2-52677H
Janelia Fluor 646
NBP2-52677JF646
PE
NBP2-52677PE
PE/Atto594
NBP2-52677PEATT594
PE/Cy5.5
NBP2-52677PECY55
PE/Cy7
NBP2-52677PECY7
PerCP
NBP2-52677PCP
Unconjugated
NBP2-52677-0.2mg
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EphA2 Antibody (1C1) [Janelia Fluor® 549] - Chimeric Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1.
Specificity
1C1 binds specifically to the amino-termianal LBD (ligand-binding domain) region of the EphA2 ectodomain (IgG1 KD ~0.8 nM; Fab KD ~140 nM) and not to other related human Eph receptors such as EphA1, EphA2, and EphA4, or any EphB receptor family members.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
EPHA2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for EphA2 Antibody (1C1) [Janelia Fluor® 549] - Chimeric

  • ARCC2
  • EC 2.7.10
  • EC 2.7.10.1
  • Eck
  • ECKepithelial cell receptor protein tyrosine kinase
  • EPH receptor A2
  • EphA2
  • ephrin type-A receptor 2
  • Epithelial cell kinase
  • Myk2
  • Sek2
  • soluble EPHA2 variant 1
  • Tyrosine-protein kinase receptor ECK

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Prolyl Oligopeptidase/PREP Antibody [NBP2-33950] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody stainin is shown in green.
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Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of FAK on different lysates using anti-FAK antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse spleen
Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Flow Cytometry: EGFR Antibody (DH8.3) - EGFRvIII [NBP2-50599] - An intracellular stain was performed on A431 cells with EGFR [DH8.3] Antibody NBP2-50599AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
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Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Src (Y419) at approximately 56 - 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of MBA‑MB‑468 human breast cancer cell line untreated (-) or treated (+) with 50 µM Pervanadate (PV) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Src (Y419) at approximately 64 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human colon using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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AKT [p Ser473]
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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-52677AF350
Alexa Fluor 405 NBP2-52677AF405
Alexa Fluor 488 NBP2-52677AF488
Alexa Fluor 532 NBP2-52677AF532
Alexa Fluor 594 NBP2-52677AF594
Alexa Fluor 647 NBP2-52677AF647
Alexa Fluor 700 NBP2-52677AF700
Alexa Fluor 750 NBP2-52677AF750
Allophycocyanin NBP2-52677APC
Allophycocyanin/Cy7 NBP2-52677APCCY7
Biotin NBP2-52677B
DyLight 350 NBP2-52677UV
DyLight 405 NBP2-52677V
DyLight 488 NBP2-52677G
DyLight 550 NBP2-52677R
DyLight 650 NBP2-52677C
DyLight 680 NBP2-52677FR
DyLight 755 NBP2-52677IR
FITC NBP2-52677F
HRP NBP2-52677H
Janelia Fluor 549 NBP2-52677JF549
Janelia Fluor 646 NBP2-52677JF646
PE NBP2-52677PE
PE/Atto594 NBP2-52677PEATT594
PE/Cy5.5 NBP2-52677PECY55
PE/Cy7 NBP2-52677PECY7
PerCP NBP2-52677PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol EPHA2