EphA2 Antibody (1C1) [Alexa Fluor® 647]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
1C1
Clonality
Monoclonal
Host
Rabbit
Conjugate
Alexa Fluor 647

Order Details

EphA2 Antibody (1C1) [Alexa Fluor® 647] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1.
Specificity
1C1 binds specifically to the amino-termianal LBD (ligand-binding domain) region of the EphA2 ectodomain (IgG1 KD ~0.8 nM; Fab KD ~140 nM) and not to other related human Eph receptors such as EphA1, EphA2, and EphA4, or any EphB receptor family members.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
EPHA2
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for EphA2 Antibody (1C1) [Alexa Fluor® 647]

  • ARCC2
  • EC 2.7.10
  • EC 2.7.10.1
  • Eck
  • ECKepithelial cell receptor protein tyrosine kinase
  • EPH receptor A2
  • EphA2
  • ephrin type-A receptor 2
  • Epithelial cell kinase
  • Myk2
  • Sek2
  • soluble EPHA2 variant 1
  • Tyrosine-protein kinase receptor ECK

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EphA2 Antibody (NBP2-52677AF647) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-52677AF350
Alexa Fluor 405 NBP2-52677AF405
Alexa Fluor 488 NBP2-52677AF488
Alexa Fluor 532 NBP2-52677AF532
Alexa Fluor 594 NBP2-52677AF594
Alexa Fluor 647 NBP2-52677AF647
Alexa Fluor 700 NBP2-52677AF700
Alexa Fluor 750 NBP2-52677AF750
Allophycocyanin NBP2-52677APC
Allophycocyanin/Cy7 NBP2-52677APCCY7
Biotin NBP2-52677B
DyLight 350 NBP2-52677UV
DyLight 405 NBP2-52677V
DyLight 488 NBP2-52677G
DyLight 550 NBP2-52677R
DyLight 650 NBP2-52677C
DyLight 680 NBP2-52677FR
DyLight 755 NBP2-52677IR
FITC NBP2-52677F
HRP NBP2-52677H
Janelia Fluor 549 NBP2-52677JF549
Janelia Fluor 646 NBP2-52677JF646
PE NBP2-52677PE
PE/Atto594 NBP2-52677PEATT594
PE/Cy5.5 NBP2-52677PECY55
PE/Cy7 NBP2-52677PECY7
PerCP NBP2-52677PCP

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Bioinformatics Tool for EphA2 Antibody (NBP2-52677AF647)

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Blogs on EphA2.

Repurposing FDA-approved drugs to combat the rise of antibiotic resistance
By Beth Melson, MSAntibiotic resistance is a global threat to public health. Widespread, inappropriate use of antibiotics, such as to treat viral infections or promote growth in livestock, has led to increased incid...  Read full blog post.

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol EPHA2