EphA2 Antibody (1C1) [DyLight 650] - Chimeric

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, CyTOF-ready, Func
• Clone: 1C1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: DyLight 650

EphA2 Antibody (1C1) [DyLight 650] - Chimeric Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: 1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1.
• Specificity: 1C1 binds specifically to the amino-termianal LBD (ligand-binding domain) region of the EphA2 ectodomain (IgG1 KD ~0.8 nM; Fab KD ~140 nM) and not to other related human Eph receptors such as EphA1, EphA2, and EphA4, or any EphB receptor family members.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EPHA2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Agonist Activity
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-52677. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for EphA2 Antibody (1C1) [DyLight 650] - Chimeric

• ARCC2
• EC 2.7.10
• EC 2.7.10.1
• Eck
• ECKepithelial cell receptor protein tyrosine kinase
• EPH receptor A2
• EphA2
• ephrin type-A receptor 2
• Epithelial cell kinase
• Myk2
• Sek2
• soluble EPHA2 variant 1
• Tyrosine-protein kinase receptor ECK

Background

Eph receptor A2 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPHA2