EphA10 Antibody (494130)

• Reactivity: Hu
• Applications: IHC
• Clone: 494130
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

EphA10 Antibody (494130) Summary

• Immunogen: E. coli-derived recombinant human EphA10
  Glu34-Glu283 (predicted)
  Accession # Q5JZY3
• Specificity: Detects human EphA10 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human EphA1, A2, A5, A6, B2, B3, B4, B6, recombinant mouse EphA3, A4, or A8 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPHA10
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphA10 Antibody (494130)

• EC 2.7.10.1
• EPH receptor A10
• EphA10
• EphA10s protein
• ephrin type-A receptor 10
• FLJ16103
• FLJ33655
• MGC43817

Background

EphA10 is a member of the Eph family of receptor tyrosine kinases that are activated by binding members of the Ephrin ligand family. EphA10 preferentially binds Ephrin-A3, -A4 and -A5.  EphA10 mRNA expression appears limited to the testis, unlike most Ephs that are expressed in developing and adult neural tissue. Human EphA10 extracellular domain (ECD) shares 95% and 88% amino acid identity with mouse and rat EphA10, respectively. Of three alternately spliced isoforms, one is truncated within the ECD and another within the cytoplasmic domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EphA10 Antibody (MAB5188)(1)

Publication using MAB5188

• WH Yang, JH Cha, W Xia, HH Lee, LC Chan, YN Wang, JL Hsu, G Ren, MC Hung Juxtacrine signaling inhibits antitumor immunity by upregulating PD-L1 expression Cancer Res., 2018-05-22;0(0):. 5/22/2018 [PMID: 29789418]

Review for EphA10 Antibody (MAB5188) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 04/04/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Breast cancer tissue
• Species: Human
• Lot: CBVQ0111101

Bioinformatics

• Gene Symbol: EPHA10
• Uniprot:
  • Q5JZY3(Human)