EpCAM/TROP1 Antibody (VU-1D9)

Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (VU-1D9) [NBP2-33051] - Immunofluorescence Analysis of MCF-7 cells EpCAM/TROP1 Antibody (VU-1D9) labeled with CF488 (green); NucSpot is used to label the nuclei (red).

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (VU-1D9) [NBP2-33051] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Ep-CAM monoclonal antibody (VU-1D9).

Flow Cytometry: EpCAM/TROP1 Antibody (VU-1D9) [NBP2-33051] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using EpCAM/TROP1 Antibody (VU-1D9).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Flow Cytometry: EpCAM/TROP1 Antibody (VU-1D9) [NBP2-33051] - Analysis using Azide and BSA Free version of NBP2-33051. Surface staining of HT29 cells using Ep-CAM MAb (VU-1D9) (red) and isotype control antibody (green).

Flow Cytometry: EpCAM/TROP1 Antibody (VU-1D9) [NBP2-33051] - Surface staining of human MCF-7 cell line with anti-human CD326 / EpCAM (VU-1D9) PerCP-CyTM5.5. Image using the PE form of this antibody.

Flow Cytometry: EpCAM/TROP1 Antibody (VU-1D9) [NBP2-33051] - Surface staining of human MCF-7 cell line with anti-human CD326 / EpCAM (VU-1D9) purified. Image using the PE form of this antibody.

Flow Cytometry: EpCAM/TROP1 Antibody (VU-1D9) [NBP2-33051] - Surface staining of Human Colon HT29 cells using EpCAM/TROP1 Antibody (VU-1D9) (red) and isotype control antibody (green).

• Reactivity: Hu, Ft(-), Rt(-)
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: VU-1D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

EpCAM/TROP1 Antibody (VU-1D9) Summary

• Immunogen: Small cell lung carcinoma cells
• Localization: Cell surface
• Marker: Epithelial Marker
• Specificity: This antibody reacts with the first EGF repeat in the extracellular domain of Ep-CAM. It is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPCAM
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-1ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug protein
• Application Notes: Human Chromosome Location: 2p21
  Mol. Weight of Antigen: 40-43kDa
  The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for EpCAM/TROP1 Antibody (VU-1D9)

• 17-1A
• 323/A3
• ACSTD1
• antigen identified by monoclonal AUA1
• CD326 antigen
• CD326
• Cell surface glycoprotein Trop-1
• chromosome 4, surface marker (35kD glycoprotein)
• DIAR5
• EGP
• EGP-2
• EGP314
• EGP40
• EpCAM
• epithelial cell adhesion molecule
• Epithelial cell surface antigen
• Epithelial glycoprotein 314
• Epithelial glycoprotein
• ESA
• GA733-2
• GA733-2EGP34
• gp40
• hEGP314
• HNPCC8
• KS 1/4 antigen
• KS1/4
• KSAHEA125
• M1S2
• M4S1
• M4S1Ly74
• Major gastrointestinal tumor-associated protein GA733-2
• MIC18MH99
• MOC31
• TACST-1
• TACSTD1
• TROP1
• TROP1CD326
• Tumor-associated calcium signal transducer 1CO-17A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EpCAM/TROP1 Antibody (NBP2-33051)(6)

Publications using NBP2-33051

• Pailler E, Faugeroux V, Oulhen M et al Acquired Resistance Mutations to ALK Inhibitors Identified by Single Circulating Tumor Cell Sequencing in ALK-Rearranged Non-Small-Cell Lung Cancer. Clin Cancer Res. 2019 Aug 22 [PMID: 31439588] (ICC/IF, Human)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Brunner A, Prelog M, Verdorfer I et al. EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder J Clin Pathol 2008 Mar [PMID: 17586680]
  
  Details:
  This reference used the PE version of NBP2-33051.
• Winter MJ, Nagtegaal ID, van Krieken JH et al. The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology Am J Pathol 2003 Dec [PMID: 14633587]
  
  Details:
  This reference used the PE version of NBP2-33051.
• Li G, Passebosc-Faure K, Lambert C et al. Flow cytometric analysis of antigen expression in malignant and normal renal cells Anticancer Res 2000 Jul [PMID: 10953356]
  
  Details:
  This reference used the PE version of NBP2-33051.
• Ogura E, Senzaki H, Yoshizawa K et al. Immunohistochemical localization of epithelial glycoprotein EGP-2 and carcinoembryonic antigen in normal colonic mucosa and colorectal tumors Anticancer Res 1998 Sep [PMID: 9854475]
  
  Details:
  This reference used the PE version of NBP2-33051.
• Tsubura A, Senzaki H, Sasaki M et al. Immunohistochemical demonstration of breast-derived and/or carcinoma-associated glycoproteins in normal skin appendages and their tumors J Cutan Pathol 1992 Feb [PMID: 1556271]
  
  Details:
  This reference used the PE version of NBP2-33051.

FAQs for EpCAM/TROP1 Antibody (NBP2-33051). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
   • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

Bioinformatics

• Gene Symbol: EPCAM