EpCAM/TROP1 Antibody (OTI1H9) - Azide and BSA Free

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Western Blot: EpCAM/TROP1 Antibody (OTI1H9) - Azide and BSA Free [NBP2-71194] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPCAM.
Immunocytochemistry/ Immunofluorescence: EpCAM/TROP1 Antibody (OTI1H9) - Azide and BSA Free [NBP2-71194] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY EPCAM.
Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) - Azide and BSA Free [NBP2-71194] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) - Azide and BSA Free [NBP2-71194] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) - Azide and BSA Free [NBP2-71194] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) - Azide and BSA Free [NBP2-71194] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) - Azide and BSA Free [NBP2-71194] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) - Azide and BSA Free [NBP2-71194] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) - Azide and BSA Free [NBP2-71194] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) - Azide and BSA Free [NBP2-71194] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) - Azide and BSA Free [NBP2-71194] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: EpCAM/TROP1 Antibody (OTI1H9) - Azide and BSA Free [NBP2-71194] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1H9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

EpCAM/TROP1 Antibody (OTI1H9) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human EPCAM(NP_002345) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
EPCAM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:4000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
Theoretical MW
32.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for EpCAM/TROP1 Antibody (OTI1H9) - Azide and BSA Free

  • 17-1A
  • 323/A3
  • ACSTD1
  • antigen identified by monoclonal AUA1
  • CD326 antigen
  • CD326
  • Cell surface glycoprotein Trop-1
  • chromosome 4, surface marker (35kD glycoprotein)
  • DIAR5
  • EGP
  • EGP-2
  • EGP314
  • EGP40
  • EpCAM
  • epithelial cell adhesion molecule
  • Epithelial cell surface antigen
  • Epithelial glycoprotein 314
  • Epithelial glycoprotein
  • ESA
  • GA733-2
  • GA733-2EGP34
  • gp40
  • hEGP314
  • HNPCC8
  • KS 1/4 antigen
  • KS1/4
  • KSAHEA125
  • M1S2
  • M4S1
  • M4S1Ly74
  • Major gastrointestinal tumor-associated protein GA733-2
  • MIC18MH99
  • MOC31
  • TACST-1
  • TACSTD1
  • TROP1
  • TROP1CD326
  • Tumor-associated calcium signal transducer 1CO-17A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EpCAM/TROP1 Antibody (NBP2-71194). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
    • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-71194AF350
Alexa Fluor 405 NBP2-71194AF405
Alexa Fluor 488 NBP2-71194AF488
Alexa Fluor 532 NBP2-71194AF532
Alexa Fluor 594 NBP2-71194AF594
Alexa Fluor 647 NBP2-71194AF647
Alexa Fluor 700 NBP2-71194AF700
Alexa Fluor 750 NBP2-71194AF750
Biotin NBP2-71194B
DyLight 350 NBP2-71194UV
DyLight 405 NBP2-71194V
DyLight 488 NBP2-71194G
DyLight 550 NBP2-71194R
DyLight 594 NBP2-71194DL594
DyLight 650 NBP2-71194C
DyLight 680 NBP2-71194FR
DyLight 755 NBP2-71194IR
FITC NBP2-71194F
HRP NBP2-71194H
Janelia Fluor 549 NBP2-71194JF549
Janelia Fluor 646 NBP2-71194JF646

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Bioinformatics Tool for EpCAM/TROP1 Antibody (NBP2-71194)

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Blogs on EpCAM/TROP1.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

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Bioinformatics

Gene Symbol EPCAM