EpCAM/TROP1 Antibody (MOC-31)

Western Blot: EpCAM/TROP1 Antibody (MOC-31) [NBP2-44640] - Western Blot analysis of human lung tissue lysate using EpCAM/TROP1 Antibody (MOC-31).

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (MOC-31) [NBP2-44640] - Human Ovarian Carcinoma stained with Ep-CAM Monoclonal Antibody (MOC-31).

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (MOC-31) [NBP2-44640] - Human Colon Carcinoma stained with Ep-CAM Monoclonal Antibody (MOC-31).

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (MOC-31) [NBP2-44640] - Human Endometrial Carcinoma stained with Ep-CAM Monoclonal Antibody (MOC-31).

SDS-Page: EpCAM/TROP1 Antibody (MOC-31) [NBP2-44640]

• Reactivity: Hu, Rt(-)
• Applications: WB, Flow, ICC/IF, IHC, IHC-P, PAGE, ICC/IF
• Clone: MOC-31
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

EpCAM/TROP1 Antibody (MOC-31) Summary

• Immunogen: Neuraminidase treated GLS-1 human small cell lung carcinoma cells
• Localization: Cell surface
• Marker: Epithelial Marker
• Specificity: Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPCAM
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100 - 1:200
  • Flow Cytometry 5 - 10 ul/million cells in 0.1ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100 - 1:200
  • SDS-Page
  • Immunofluorescence 1:50 - 1:100
• Application Notes: Hu-chromosome location: 2q21
  Molecular weight of antigen: 40-43kDa
  Immunohistochemistry-Paraffin 1:100 - 1:200 for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 28139197). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for EpCAM/TROP1 Antibody (MOC-31)

• 17-1A
• 323/A3
• ACSTD1
• antigen identified by monoclonal AUA1
• CD326 antigen
• CD326
• Cell surface glycoprotein Trop-1
• chromosome 4, surface marker (35kD glycoprotein)
• DIAR5
• EGP
• EGP-2
• EGP314
• EGP40
• EpCAM
• epithelial cell adhesion molecule
• Epithelial cell surface antigen
• Epithelial glycoprotein 314
• Epithelial glycoprotein
• ESA
• GA733-2
• GA733-2EGP34
• gp40
• hEGP314
• HNPCC8
• KS 1/4 antigen
• KS1/4
• KSAHEA125
• M1S2
• M4S1
• M4S1Ly74
• Major gastrointestinal tumor-associated protein GA733-2
• MIC18MH99
• MOC31
• TACST-1
• TACSTD1
• TROP1
• TROP1CD326
• Tumor-associated calcium signal transducer 1CO-17A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EpCAM/TROP1 Antibody (NBP2-44640)(1)

Publication using NBP2-44640

• Franco-Barraza J, Francescone R, Luong T et al. Matrix-regulated integrin alphavb5 maintains alpha5b1-dependent desmoplastic traits prognostic of neoplastic recurrence. Elife. 2017 Jan 31 [PMID: 28139197] (ICC/IF, Human)

FAQs for EpCAM/TROP1 Antibody (NBP2-44640). (Showing 1 - 1 of 1 FAQ).

1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
   • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

Bioinformatics

• Gene Symbol: EPCAM
• Uniprot:
  • P16422()