EpCAM/TROP1 Antibody (EGP40/826)

Images

 
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/826) [NBP2-44643] - Human Breast Carcinoma stained with Ep-CAM Monoclonal Antibody (EGP40/826).
Immunofluorescence: EpCAM/TROP1 Antibody (EGP40/826) [NBP2-44643] - Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled EpCAM Monoclonal Antibody (EGP40/826) (Green). F-actin filaments were labeled ...read more
Immunofluorescence: EpCAM/TROP1 Antibody (EGP40/826) [NBP2-44643] - Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG1) (Green). DAPI was used to stain the ...read more
SDS-Page: EpCAM/TROP1 Antibody (EGP40/826) [NBP2-44643]

Product Details

Summary
Reactivity Hu, Mu(-), Rt(-)Species Glossary
Applications WB, Flow, IHC, IHC-P, PAGE, ICC/IF
Clone
EGP40/826
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

EpCAM/TROP1 Antibody (EGP40/826) Summary

Immunogen
A synthetic peptide (around aa 20-60) from the N-terminus of human TACSTD1 protein.
Localization
Cell surface
Marker
Epithelial Marker
Specificity
Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EPCAM
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • SDS-Page
  • Western Blot 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 2q21
Molecular weight of antigen: 40-43kDa
Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for EpCAM/TROP1 Antibody (EGP40/826)

  • 17-1A
  • 323/A3
  • ACSTD1
  • antigen identified by monoclonal AUA1
  • CD326 antigen
  • CD326
  • Cell surface glycoprotein Trop-1
  • chromosome 4, surface marker (35kD glycoprotein)
  • DIAR5
  • EGP
  • EGP-2
  • EGP314
  • EGP40
  • EpCAM
  • epithelial cell adhesion molecule
  • Epithelial cell surface antigen
  • Epithelial glycoprotein 314
  • Epithelial glycoprotein
  • ESA
  • GA733-2
  • GA733-2EGP34
  • gp40
  • hEGP314
  • HNPCC8
  • KS 1/4 antigen
  • KS1/4
  • KSAHEA125
  • M1S2
  • M4S1
  • M4S1Ly74
  • Major gastrointestinal tumor-associated protein GA733-2
  • MIC18MH99
  • MOC31
  • TACST-1
  • TACSTD1
  • TROP1
  • TROP1CD326
  • Tumor-associated calcium signal transducer 1CO-17A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-44643
Species: Hu, Mu(-), Rt(-)
Applications: WB, Flow, IHC, IHC-P, PAGE, ICC/IF

Publications for EpCAM/TROP1 Antibody (NBP2-44643) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for EpCAM/TROP1 Antibody (NBP2-44643). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
    • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-47876AF405
Alexa Fluor 488 NBP2-47876AF488
Alexa Fluor 647 NBP2-47876AF647
Alexa Fluor 700 NBP2-47876AF700
Allophycocyanin NBP2-47876APC
Allophycocyanin/Cy7 NBP2-47876APCCY7
Biotin NBP2-47876B
DyLight 350 NBP2-47876UV
DyLight 405 NBP2-47876V
DyLight 488 NBP2-47876G
DyLight 550 NBP2-47876R
DyLight 650 NBP2-47876C
DyLight 680 NBP2-47876FR
DyLight 755 NBP2-47876IR
FITC NBP2-47876F
HRP NBP2-47876H
Janelia Fluor 549 NBP2-47876JF549
Janelia Fluor 646 NBP2-47876JF646
PE NBP2-47876PE
PE/Atto594 NBP2-47876PEATT594
PE/Cy5.5 NBP2-47876PECY55
PE/Cy7 NBP2-47876PECY7
PerCP NBP2-47876PCP

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Bioinformatics Tool for EpCAM/TROP1 Antibody (NBP2-44643)

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Blogs on EpCAM/TROP1.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

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Bioinformatics

Gene Symbol EPCAM
Uniprot