EpCAM/TROP1 Antibody (EGP40/1110)

Images

 
Western Blot: EpCAM/TROP1 Antibody (EGP40/1110) [NBP2-44635]
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/1110) [NBP2-44635] - Rat Oviduct stained with Ep-CAM Monoclonal Antibody (EGP40/1110).
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/1110) [NBP2-44635] - Human Ovarian Carcinoma stained with Ep-CAM Monoclonal Antibody (EGP40/1110).
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/1110) [NBP2-44635] - Rat Colon stained with Ep-CAM Monoclonal Antibody (EGP40/1110).
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/1110) [NBP2-44635] - Rat Lung stained with Ep-CAM Monoclonal Antibody (EGP40/1110).
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (EGP40/1110) [NBP2-44635] - Formalin-fixed, paraffin-embedded Mouse Colon stained with EpCAM Mouse Monoclonal Antibody (EGP40/1110).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC-P, ICC/IF
Clone
EGP40/1110
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

EpCAM/TROP1 Antibody (EGP40/1110) Summary

Immunogen
A recombinant human EpCAM fragment from the cytoplasmic domain (around aa 280-350) (exact sequence is proprietary)
Localization
Cell surface
Marker
Epithelial Marker
Specificity
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EPCAM
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.0 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 1 - 2 ug/ml
Application Notes
Hu-chromosome location: 2p21
Molecular weight of antigen: 40-43kDa
Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for EpCAM/TROP1 Antibody (EGP40/1110)

  • 17-1A
  • 323/A3
  • ACSTD1
  • antigen identified by monoclonal AUA1
  • CD326 antigen
  • CD326
  • Cell surface glycoprotein Trop-1
  • chromosome 4, surface marker (35kD glycoprotein)
  • DIAR5
  • EGP
  • EGP-2
  • EGP314
  • EGP40
  • EpCAM
  • epithelial cell adhesion molecule
  • Epithelial cell surface antigen
  • Epithelial glycoprotein 314
  • Epithelial glycoprotein
  • ESA
  • GA733-2
  • GA733-2EGP34
  • gp40
  • hEGP314
  • HNPCC8
  • KS 1/4 antigen
  • KS1/4
  • KSAHEA125
  • M1S2
  • M4S1
  • M4S1Ly74
  • Major gastrointestinal tumor-associated protein GA733-2
  • MIC18MH99
  • MOC31
  • TACST-1
  • TACSTD1
  • TROP1
  • TROP1CD326
  • Tumor-associated calcium signal transducer 1CO-17A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

FAQs for EpCAM/TROP1 Antibody (NBP2-44635). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
    • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

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Bioinformatics

Gene Symbol EPCAM
Uniprot