EpCAM/TROP1 Antibody (323/A3) [Alexa Fluor® 488] - (ECD)

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Product Details

Summary
Reactivity Hu, Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
323/A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

Order Details

EpCAM/TROP1 Antibody (323/A3) [Alexa Fluor® 488] - (ECD) Summary

Immunogen
MCF-7 human breast cancer cells
Localization
Cell surface
Marker
Epithelial Marker
Specificity
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EPCAM
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Does not react with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for EpCAM/TROP1 Antibody (323/A3) [Alexa Fluor® 488] - (ECD)

  • 17-1A
  • 323/A3
  • ACSTD1
  • antigen identified by monoclonal AUA1
  • CD326 antigen
  • CD326
  • Cell surface glycoprotein Trop-1
  • chromosome 4, surface marker (35kD glycoprotein)
  • DIAR5
  • EGP
  • EGP-2
  • EGP314
  • EGP40
  • EpCAM
  • epithelial cell adhesion molecule
  • Epithelial cell surface antigen
  • Epithelial glycoprotein 314
  • Epithelial glycoprotein
  • ESA
  • GA733-2
  • GA733-2EGP34
  • gp40
  • hEGP314
  • HNPCC8
  • KS 1/4 antigen
  • KS1/4
  • KSAHEA125
  • M1S2
  • M4S1
  • M4S1Ly74
  • Major gastrointestinal tumor-associated protein GA733-2
  • MIC18MH99
  • MOC31
  • TACST-1
  • TACSTD1
  • TROP1
  • TROP1CD326
  • Tumor-associated calcium signal transducer 1CO-17A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Rt(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-P

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EpCAM/CD326 Antibody (NBP2-34532AF488). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
    • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34532AF350
Alexa Fluor 405 NBP2-34532AF405
Alexa Fluor 488 NBP2-34532AF488
Alexa Fluor 532 NBP2-34532AF532
Alexa Fluor 594 NBP2-34532AF594
Alexa Fluor 647 NBP2-34532AF647
Alexa Fluor 700 NBP2-34532AF700
Alexa Fluor 750 NBP2-34532AF750
Allophycocyanin NBP2-34532APC
Allophycocyanin/Cy7 NBP2-34532APCCY7
Biotin NBP2-34532B
DyLight 350 NBP2-34532UV
DyLight 405 NBP2-34532V
DyLight 488 NBP2-34532G
DyLight 550 NBP2-34532R
DyLight 594 NBP2-34532DL594
DyLight 650 NBP2-34532C
DyLight 680 NBP2-34532FR
DyLight 755 NBP2-34532IR
FITC NBP2-34532F
HRP NBP2-34532H
Janelia Fluor 549 NBP2-34532JF549
Janelia Fluor 646 NBP2-34532JF646
PE NBP2-34532PE
PE/Atto594 NBP2-34532PEATT594
PE/Cy5.5 NBP2-34532PECY55
PE/Cy7 NBP2-34532PECY7

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Bioinformatics Tool for EpCAM/CD326 Antibody (NBP2-34532AF488)

Discover related pathways, diseases and genes to EpCAM/CD326 Antibody (NBP2-34532AF488). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on EpCAM/TROP1.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

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Bioinformatics

Gene Symbol EPCAM