EOMES Antibody (CL15425) - Azide and BSA Free

Images

 
Orthogonal Strategies: Analysis in human tonsil and prostate tissues using NBP3-44155 antibody. Corresponding EOMES RNA-seq data are presented for the same tissues.
Staining of human cerebellum shows strong nuclear positivity in a subset of neurons in the granular layer.
Staining of human prostate shows no positivity in glandular cells as expected.
Staining of mouse brain shows moderate to strong nuclear positivity in a subset of neurons in the cerebellum.
Staining of mouse embryo, E14 shows moderate to strong nuclear positivity in neuronal cells in the developing cortex.
Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications IHC
Clone
CL15425
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH
Validated by:
       

Orthogonal Strategies

 

Order Details

EOMES Antibody (CL15425) - Azide and BSA Free Summary

Immunogen
This antibody was generated using a recombinant protein sequence of O95936, with the exact immunogen sequence remaining proprietary.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EOMES
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for EOMES Antibody (CL15425) - Azide and BSA Free

  • EOMES
  • eomesodermin (Xenopus laevis) homolog
  • Eomesodermin
  • t box, brain, 2
  • T-box brain protein 2
  • T-box brain2
  • TBR2
  • TBR-2
  • TBR2eomesodermin homolog
  • T-brain-2

Background

EOMES encodes a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar gene disrupted in mice is shown to be essential during trophoblast development and gastrulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EOMES