ENOSF1 Antibody

Images

 
Western Blot: ENOSF1 Antibody [NBP2-16338] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: ENOSF1 Antibody [NBP2-16338] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: ENOSF1 Antibody [NBP2-16338] - Immunohistochemical analysis of paraffin-embedded gastric ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ENOSF1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ENOSF1. The exact sequence is proprietary.
Localization
Mitochondrion
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ENOSF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Bovine (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for ENOSF1 Antibody

  • Antisense RNA to thymidylate synthase
  • EC 5.-
  • enolase superfamily member 1
  • HSRTSBETA
  • rTS beta
  • rTS
  • RTSrTS alpha
  • TYMSASmitochondrial enolase superfamily member 1

Background

This gene was originally identified as a naturally occurring antisense transcript to the human thymidylate synthase gene. Alternate splice variants have been described, one of which (named rTSalpha) represents an alternate 3'UTR that is complementary to the 3'UTR and terminal intron of the thymidylate synthase (TS) RNA and down-regulates TS expression. Another transcript variant (named rTSalpha) does not overlap the TS locus. The function of this gene appears to be primarily to regulate expression of the TS locus both via the antisense transcript as well as through the encoded proteins. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Diseases for ENOSF1 Antibody (NBP2-16338)

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Calculators

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Bioinformatics

Gene Symbol ENOSF1
Uniprot