RECQ1 Antibody

Images

 
Biological Strategies: Western Blot: RECQ1 Antibody [NB100-619] - Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-RecQ1 antibody NB100-619 used ...read more
Immunohistochemistry: RECQ1 Antibody [NB100-619] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RecQ1 at 1:5000 (0.2 ug/mL). Detection: DAB
Independent Antibodies: Immunoprecipitation: RECQ1 Antibody [NB100-619] - Detection of human RecQ1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP ...read more

Product Details

Summary
Reactivity Hu, Pm-OrSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

RECQ1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 600 and the C-terminus (residue 649) of human RecQ protein-like (DNA helicase Q1-like) 1 using the numbering given in Swiss-Prot entry P46063 (GeneID 5965).
Predicted Species
Orangutan (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RECQL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:2000-1:10000
  • Immunoprecipitation 2-10 ug/mg lysate
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
RECQ1 Overexpression Lysate
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB100-619 in the following applications:

Publications
Read Publications using
NB100-619 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RECQ1 Antibody

  • DNA helicase, RecQ-like type 1
  • DNA-dependent ATPase Q1
  • EC 3.6.1
  • EC 3.6.4.12
  • RecQ protein-like (DNA helicase Q1-like)
  • RecQ protein-like 1
  • RECQ1
  • RecQ1ATP-dependent DNA helicase Q1
  • RecQL1

Background

The protein encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Some members of this family are associated with genetic disorders with predisposition to malignancy and chromosomal instability. The biological function of this helicase has not yet been determined. Two alternatively spliced transcripts, which encode the same isoform but differ in their 5' and 3' UTRs, have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-214
Species: Hu, Mu
Applications: WB, IP
NB100-471
Species: Hu
Applications: WB, ICC/IF, IHC, IP, ChIP
NBP2-47310
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-83438
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
NB100-148
Species: Hu, Mu, Rt, Ce, Ch
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, PLA, KD
NBP1-83077
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
NBP3-04913
Species: Hu, Mu, Rt
Applications: WB
NBP2-16338
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB600-1101
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-2204
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NBP2-32016
Species: Hu
Applications: IHC, IHC-P
NBP2-67219
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NB100-56599
Species: Hu, Mu, Rt(-)
Applications: WB, Flow, ICC/IF, Flow-IC
NBP2-33916
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-31883
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-309
Species: Hu, Mu, Rt, Pm
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, KD
NB100-150
Species: Hu, Mu
Applications: WB, ICC/IF, IP
H00007520-M02
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA

Publications for RECQ1 Antibody (NB100-619)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for RECQ1 Antibody (NB100-619) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NB100-619:
Filter by Applications
IP
(1)
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Immunoprecipitation RECQ1 NB100-619
Enlarge
5
reviewed by:
Anonymous
IP Human 12/04/2018
View

Summary

ApplicationImmunoprecipitation
Sample Testedb-cell
SpeciesHuman
LotA2

Comments

Comments10ug/mg lysate
Immunocytochemistry RECQ1 NB100-619
Enlarge
4
reviewed by:
Anonymous
ICC Human 03/13/2018
View

Summary

ApplicationImmunocytochemistry
Sample Tested fibroblasts
SpeciesHuman
LotA2

Comments

CommentsBIOSCARF

1:250 in 1% BSA/0.3% Triton X-100 / PBS
Western Blot RECQ1 NB100-619
Enlarge
4
reviewed by:
Anonymous
WB Human 12/12/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman lymphocytes, whole cell lysate
SpeciesHuman
LotA2

Comments

Comments25ug protein, 10 second exposure. 1:2000 dilution in 5% milk/TBS-T. Longer exposure (1 min had several bands).

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RECQ1 Antibody (NB100-619) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional RECQ1 Products

Bioinformatics Tool for RECQ1 Antibody (NB100-619)

Discover related pathways, diseases and genes to RECQ1 Antibody (NB100-619). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for RECQ1 Antibody (NB100-619)

Discover more about diseases related to RECQ1 Antibody (NB100-619).
 

Pathways for RECQ1 Antibody (NB100-619)

View related products by pathway.

PTMs for RECQ1 Antibody (NB100-619)

Learn more about PTMs related to RECQ1 Antibody (NB100-619).

Blogs on RECQ1

There are no specific blogs for RECQ1, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Anonymous
12/04/2018
Application: IP
Species: Human

Anonymous
03/13/2018
Application: ICC
Species: Human

Anonymous
12/12/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol RECQL
Entrez
Uniprot