eNOS Antibody - Pre-diluted

Product Discontinued

eNOS Antibody - Pre-diluted Summary

• Immunogen: Synthetic peptide (Human) (C terminal).
• Localization: Secreted
• Specificity: eNOS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOS3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin Neat
• Application Notes: Immunocytochemistry/Immunoflourescence was reported in the literature. (PMID 22363674)
• Theoretical MW: 135 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Monkey cross reactivity was reported in the literature (PMID: 22363674) Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 7.0 ml pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1% BSA buffer pH 7.6.
• Preservative: 0.1% Sodium Azide
• Purity: Immunogen affinity purified

Notes

7mL size is pre-diluted and this product can be used Neat (without further dilution). Use recommended dilution for the concentrated vials only.

Alternate Names for eNOS Antibody - Pre-diluted

• Constitutive NOS
• EC 1.14.13.39
• ECNOS
• Endothelial NOS
• endothelial
• eNOS
• eNOScNOS
• nitric oxide synthase 3 (endothelial cell)
• NOS type III
• NOS3
• NOSIII

Background

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eNOS Antibody (NB120-15280)(3)

Publications using NB120-15280

• Garcia V, Park E. J, et al. Unbiased proteomics identifies plasminogen activator inhibitor-1 as a negative regulator of endothelial nitric oxide synthase. Proc Natl Acad Sci U S A 2020-04-28 [PMID: 32300005]
• Park JK, Utsumi T, Seo YE et al. Cellular distribution of injected PLGA-nanoparticles in the liver Nanomedicine 2016-03-04 [PMID: 26961463] (ICC/IF, Mouse)
• Sangwung P, Greco TM, Wang Y, Ischiropoulos H, Sessa WC, Iwakiri Y. Proteomic Identification of S-Nitrosylated Golgi Proteins: New Insights into Endothelial Cell Regulation by eNOS-Derived NO. PLoS One;7(2):e31564. 2012-01-01 [PMID: 22363674]

Bioinformatics

• Gene Symbol: NOS3
• Entrez:
  • 4846(Human)
  • 18127(Mouse)
  • 24600(Rat)