Enolase 2/Neuron-specific Enolase Antibody

Immunohistochemistry-Frozen: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090] - Frozen sectioned E13.5 Rat brain. Green: NSE protein stained by NSE antibody [N1C1] diluted at 1:250. Blue: Fluoroshield with DAPI.

Western Blot: Enolase 1 Antibody [NBP1-32090] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE; antibody diluted at 1:10000.

Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP1-32090] - Paraffin-embedded PC14 xenograft, using antibody at 1:100 dilution.

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 2/Neuron-specific Enolase antibody [N1C1] (NBP1-32090) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NBP1-32090] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 2/Neuron-specific Enolase antibody [N1C1] (NBP1-32090) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Enolase 2/Neuron-specific Enolase Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Enolase 2/Neuron-specific Enolase. The exact sequence is proprietary.
• Localization: Cytoplasm , Cell membrane
• Predicted Species: Bovine (98%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ENO2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (83%), Chicken (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody

• 2-phospho-D-glycerate hydrolyase
• 2-phospho-D-glycerate hydro-lyase
• EC 4.2.1.11
• ENO2
• enolase 2 (gamma, neuronal)
• Enolase 2
• gamma-Enolase
• Neural enolase
• neuron specific gamma enolase
• neurone-specific enolase
• Neuronspecific Enolase
• Neuron-specific enolase
• NSE

Background

This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ENO2