Enolase 1 Antibody - BSA Free

Western Blot: Enolase 1 Antibody [NBP1-31324] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP1-31324] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ENO1 protein stained by ENO1 antibody [N3C3] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP1-31324] - Paraffin-embedded gastric tissue, using antibody at 1:100 dilution.

Western Blot: Enolase 1 Antibody [NBP1-31324] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.

Western Blot: Enolase 1 Antibody [NBP1-31324] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE, antibody diluted at 1:3000.

Western Blot: Enolase 1 Antibody [NBP1-31324] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3].

Western Blot: Enolase 1 Antibody [NBP1-31324] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: Enolase 1 Antibody [NBP1-31324] - Sample (30 ug of whole cell lysate) A: zebrafish eye 10% SDS PAGE NBP1-31324 diluted at 1:1000

Immunohistochemistry: Enolase 1 Antibody [NBP1-31324] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Enolase 1 antibody [N3C3] (NBP1-31324) at 1:300 dilution.

Immunohistochemistry: Enolase 1 Antibody [NBP1-31324] - Enolase 1 antibody [N3C3] detects Eno1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. Enolase 1 antibody [N3C3] (NBP1-31324) dilution: 1:100.

• Reactivity: Hu, Mu, Rt, Ze, Bv, Ch, Pm, Gp, RM, Xp
• Applications: WB, DB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Enolase 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Enolase 1 Antibody - BSA Free (NBP1-31324) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Enolase 1. The exact sequence is proprietary.
• Localization: Cytoplasm, Cell membrane, myofibril, sarcomere, M-band, Isoform MBP-1: Nucleus
• Predicted Species: Xenopus (90%), Chicken (93%), Chimpanzee (100%), Bovine (93%), Guinea Pig (95%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ENO1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:20000
• Application Notes: Dot Blot-Assay dependent
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (90%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Enolase 1 Antibody - BSA Free

• 2-phospho-D-glycerate hydro-lyase
• Alpha enolase
• C-myc promoter-binding protein
• c-myc promoter-binding protein-1
• EC 4.2.1
• EC 4.2.1.11
• ENO1L1
• Enolase 1
• enolase 1, (alpha)
• MBP-1
• MBPB1
• MPB-1
• MPB1c-myc promoter-binding protein 1
• MYC promoter-binding protein 1
• NNE
• Non-neural enolase
• Phosphopyruvate hydratase
• Plasminogen-binding protein
• PPHalpha enolase like 1
• tau-crystallin

Background

This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ENO1