Endosialin/CD248/TEM1 Antibody (458606)

Endosialin/CD248 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse Endosialin/CD248 Monoclonal Antibody (Catalog # MAB7535) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Endosialin/CD248 was detected in immersion fixed IMR-32 human neuroblastoma cell line using Rat Anti-Mouse Endosialin/CD248 Monoclonal Antibody (Catalog # MAB7535) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Mu
• Applications: ICC/IF
• Clone: 458606
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Endosialin/CD248/TEM1 Antibody (458606) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Endosialin/CD248
  Val13-Arg693 (predicted)
  Accession # Q91V98
• Specificity: Detects mouse Endosialin/CD248 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human Endosialin/CD248 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CD248
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endosialin/CD248/TEM1 Antibody (458606)

• 2610111G01Rik
• CD164 sialomucin-like 1
• CD164L1
• CD248 antigen
• CD248 antigen, endosialin
• CD248 molecule, endosialin
• CD248
• Endosialin
• MGC119479
• TEM1
• TEM1MGC119478
• Tumor endothelial marker 1

Background

Endosialin, also known as CD248 and tumor endothelial marker 1 (Tem1), is a 165 kDa transmembrane O-glycosylated protein that contains one C-type lectin, one Sushi, one EGF-like domain and a mucin-like stalk in its extracellular domain (ECD). It is expressed on activated perivascular and stromal cells in embyronic and tumor neovasculature but is downregulated in quiescent vasculature. Endosialin regulates pericyte proliferation, migration, and adhesion to matrix Fibronectin and Collagens I and IV. It is also required for lymph node and spleen remodeling during immune responses. Within the ECD, mouse Endosialin shares 76% and 93% aa sequence identity with human and rat Endosialin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD248
• Uniprot:
  • Q91V98(Mouse)