Endo G Antibody (OTI5F1) - Azide and BSA Free

Western Blot: Endo G Antibody (OTI5F1) - Azide and BSA Free [NBP2-70645] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Endo G (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Endo G.

Immunocytochemistry/Immunofluorescence: Endo G Antibody (OTI5F1) - Azide and BSA Free [NBP2-70645] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Endo G.

Immunohistochemistry: Endo G Antibody (OTI5F1) - Azide and BSA Free [NBP2-70645] - Staining of paraffin-embedded Human pancreas tissue using anti-Endo G mouse monoclonal antibody.

Flow Cytometry: Endo G Antibody (OTI5F1) - Azide and BSA Free [NBP2-70645] - Analysis of Jurkat cells, using anti-Endo G antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry: Endo G Antibody (OTI5F1) - Azide and BSA Free [NBP2-70645] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Endo G mouse monoclonal antibody.

Immunohistochemistry: Endo G Antibody (OTI5F1) - Azide and BSA Free [NBP2-70645] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Endo G mouse monoclonal antibody.

Immunohistochemistry: Endo G Antibody (OTI5F1) - Azide and BSA Free [NBP2-70645] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Endo G mouse monoclonal antibody.

Immunohistochemistry: Endo G Antibody (OTI5F1) - Azide and BSA Free [NBP2-70645] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Endo G mouse monoclonal antibody.

Immunohistochemistry: Endo G Antibody (OTI5F1) - Azide and BSA Free [NBP2-70645] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Endo G mouse monoclonal antibody.

Immunohistochemistry: Endo G Antibody (OTI5F1) - Azide and BSA Free [NBP2-70645] - Staining of paraffin-embedded Human Kidney tissue using anti-Endo G mouse monoclonal antibody.

Immunohistochemistry: Endo G Antibody (OTI5F1) - Azide and BSA Free [NBP2-70645] - Staining of paraffin-embedded Human liver tissue using anti-Endo G mouse monoclonal antibody.

Immunohistochemistry: Endo G Antibody (OTI5F1) - Azide and BSA Free [NBP2-70645] - Staining of paraffin-embedded Human tonsil using anti-Endo G mouse monoclonal antibody.

Flow Cytometry: Endo G Antibody (OTI5F1) - Azide and BSA Free [NBP2-70645] - Analysis of Hela cells, using anti-Endo G antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI5F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Endo G Antibody (OTI5F1) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human ENDOG(NP_004426) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENDOG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 27.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Endo G Antibody (OTI5F1) - Azide and BSA Free

• EC 3.1.30.-
• Endo G
• endonuclease G
• endonuclease G, mitochondrial
• FLJ27463
• mitochondrial endonuclease G

Background

The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1,2). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndooG also cleaves DNA in vitro (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENDOG