EMMPRIN/CD147 Antibody (OX-47) - BSA Free

Flow Cytometry: EMMPRIN/CD147 Antibody (OX-47) [NB100-64848] - Rat Splenocytes were stained with EMMPRIN/CD147 (OX-47) antibody NB100-64848 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Fluor 488.

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (OX-47) [NB100-64848] - Analysis of FFPE tissue section of rat kidney using 1:100 dilution of CD147 antibody (clone OX-47). The staining was detected using HRP-labelled secondary antibody and DAB reagent which followed counterstaining of sections with hematoxylin. This antibody generated primarily a membrane staining with some cytoplasmic signal in the cuboidal epithelial cells of various tubules.

• Reactivity: Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OX-47
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

EMMPRIN/CD147 Antibody (OX-47) - BSA Free Summary

• Immunogen: T blasts from a mixed lymphocyte reaction.
• Specificity: NB100-64848 recognizes a membrane glycoprotein of MW 42-48 kD expressed at high levels on activated lymphocytes but lower levels on resting cells. The antigen is also present on some endothelia. The antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue. It is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BSG
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Frozen 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Western Blot 1:100 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for EMMPRIN/CD147 Antibody (OX-47) - BSA Free

• 5F7
• basigin (Ok blood group)
• Basigin
• BSG
• CD147 antigen
• CD147
• Collagenase stimulatory factor
• EMMPRIN
• EMMPRINTCSF
• Extracellular matrix metalloproteinase inducer
• Leukocyte activation antigen M6
• M6
• OK blood group antigen
• OK
• Tumor cell-derived collagenase stimulatory factor

Background

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EMMPRIN/CD147 Antibody (NB100-64848)(6)

Publications using NB100-64848

• Jin R, Xiao AY, Liu S et al. Taurine Reduces tPA (Tissue-Type Plasminogen Activator)-Induced Hemorrhage and Microvascular Thrombosis After Embolic Stroke in Rat Stroke 2018-07-01 [PMID: 29844028] (WB, Rat)
• Foda, H et al. Ventilator-induced lung injury upregulates activates gelatinases EMMPRIN. Am. J. Respir. Cell. Mol. Biol. 25: 717 - 724. 2001-01-01 [PMID: 11726397]
• Crook K, Hunt SV. Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82. Dev Immunol. [PMID: 8924759] (FLOW, Rat)
• Cassella, JP et al. Ontogeny of four blood-brain barrier markers: an immunocytochemical comparison of pial cerebral cortical microvessels. J. Anat. 189: 407-15. 1996-01-01 [PMID: 8886962]
• Paterson, DJ, Jefferies WA, Green, JR et al. Antigens of Activated rat T Lymphocytes including a molecule of 50,000 Mr detected only by CD4 positive T blasts. Mol. Immunol. 24: 1281. 1987-01-01 [PMID: 2828930]
• Fossum S, Mallett S amp Barclay AN. The MRC OX-47 antigen is a member of the immunoglobulin superfamily with an unusual transmembrane sequencequot;. Eur. J. Immunol. 21: 671. 1991-01-01 [PMID: 2009910]

Bioinformatics

• Gene Symbol: BSG
• Entrez:
  • 25246(Rat)
• Uniprot:
  • P26453(Rat)