EMMPRIN/CD147 Antibody (OX-47)

Images

 
Flow Cytometry: EMMPRIN/CD147 Antibody (OX-47) [NB100-64848] - Rat Splenocytes were stained with EMMPRIN/CD147 (OX-47) antibody NB100-64848 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated ...read more
Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (OX-47) [NB100-64848] - Analysis of FFPE tissue section of rat kidney using 1:100 dilution of CD147 antibody (clone OX-47). The staining was detected using ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
OX-47
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

EMMPRIN/CD147 Antibody (OX-47) Summary

Immunogen
T blasts from a mixed lymphocyte reaction.
Specificity
NB100-64848 recognizes a membrane glycoprotein of MW 42-48 kD expressed at high levels on activated lymphocytes but lower levels on resting cells. The antigen is also present on some endothelia. The antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue. It is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BSG
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Flow Cytometry 1 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Frozen 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • CyTOF-ready
Control
EMMPRIN/CD147 Knockout 293T Cell Lysate
Publications
Read Publications using
NB100-64848 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for EMMPRIN/CD147 Antibody (OX-47)

  • 5F7
  • basigin (Ok blood group)
  • Basigin
  • BSG
  • CD147 antigen
  • CD147
  • Collagenase stimulatory factor
  • EMMPRIN
  • EMMPRINTCSF
  • Extracellular matrix metalloproteinase inducer
  • Leukocyte activation antigen M6
  • M6
  • OK blood group antigen
  • OK
  • Tumor cell-derived collagenase stimulatory factor

Background

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready

Publications for EMMPRIN/CD147 Antibody (NB100-64848)(6)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EMMPRIN/CD147 Antibody (NB100-64848) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-64848AF350
Alexa Fluor 405 NB100-64848AF405
Alexa Fluor 488 NB100-64848AF488
Alexa Fluor 532 NB100-64848AF532
Alexa Fluor 594 NB100-64848AF594
Alexa Fluor 647 NB100-64848AF647
Alexa Fluor 700 NB100-64848AF700
Alexa Fluor 750 NB100-64848AF750
Allophycocyanin NB100-64848APC
Biotin NB100-64848B
DyLight 350 NB100-64848UV
DyLight 405 NB100-64848V
DyLight 488 NB100-64848G
DyLight 550 NB100-64848R
DyLight 594 NB100-64848DL594
DyLight 650 NB100-64848C
DyLight 680 NB100-64848FR
DyLight 755 NB100-64848IR
FITC NB100-64848F
HRP NB100-64848H
Janelia Fluor 549 NB100-64848JF549
Janelia Fluor 646 NB100-64848JF646
PE NB100-64848PE
PerCP NB100-64848PCP

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Bioinformatics Tool for EMMPRIN/CD147 Antibody (NB100-64848)

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Bioinformatics

Gene Symbol BSG
Entrez
Uniprot