EMMPRIN/CD147 Antibody (MEM-M6/2) - BSA Free

Flow (Cell Surface): EMMPRIN/CD147 Antibody (MEM-M6/2) [NB500-561] - Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/2) [FITC] [NBP1-44585] - Surface staining of human peripheral blood with anti-human CD147 (MEM-M6/1) FITC. Image using the FITC form of this antibody.

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/2) [NB500-561] - Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/2) [FITC] [NBP1-44585] - Surface staining of human peripheral blood with anti-human CD147 (MEM-M6/1) biotin. Image using the FITC form of this antibody.

Surface staining pattern of human peripheral whole blood stained using anti-human CD147 (MEM-M6/2) purified antibody (concentration in sample 0.6 ug/ml, GAM APC).

• Reactivity: Hu, Po
• Applications: Flow, IHC, CyTOF-ready
• Clone: MEM-M6/2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

EMMPRIN/CD147 Antibody (MEM-M6/2) - BSA Free Summary

• Description: Novus Biologicals Mouse EMMPRIN/CD147 Antibody (MEM-M6/2) - BSA Free (NB500-561) is a monoclonal antibody validated for use in IHC and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the entire extracellular region of CD147 fused to the DNA coding for the hinge region, CH2 and CH3 domain of human IgG1.
• Epitope: This antibody recognises extracellular Ig domain D1 of CD147 antigen.
• Localization: Cell Membrane
• Specificity: The antibody MEM-M6/2 recognizes extracellular Ig domain D1 of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: BSG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  EMMPRIN/CD147 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for EMMPRIN/CD147 Antibody (MEM-M6/2) - BSA Free

• 5F7
• basigin (Ok blood group)
• Basigin
• BSG
• CD147 antigen
• CD147
• Collagenase stimulatory factor
• EMMPRIN
• EMMPRINTCSF
• Extracellular matrix metalloproteinase inducer
• Leukocyte activation antigen M6
• M6
• OK blood group antigen
• OK
• Tumor cell-derived collagenase stimulatory factor

Background

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BSG