EMMPRIN/CD147 Antibody (JF1-045)

Immunocytochemistry/Immunofluorescence: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Staining CD147 in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Analysis of paraffin-embedded rat brain tissue using anti-CD147 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Staining CD147 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunocytochemistry/Immunofluorescence: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Staining CD147 in HeLa cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Analysis of paraffin-embedded human colon cancer tissue using anti-CD147 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Analysis of paraffin-embedded mouse brain tissue using anti-CD147 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Analysis of paraffin-embedded mouse heart tissue using anti-CD147 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - Analysis of paraffin-embedded mouse testis tissue using anti-CD147 antibody. Counter stained with hematoxylin.

Western Blot: EMMPRIN/CD147 Antibody (JF1-045) [NBP2-66820] - analysis of CD147 on different lysates with Rabbit anti-CD147 antibody at 1/5,000 dilution.Lane 1: HepG2 cell lysate (15 ug/Lane)Lane 2: NCI-H226 cell lysate (15 ug/Lane)Lane 3: 786-0 cell lysate (15 µg/Lane)Lane 4: RAW264.7 cell lysate (15 ug/Lane)Lane 5: Mouse liver tissue lysate (20 ug/Lane)Lane 6: Rat liver tissue lysate (20 ug/Lane)Predicted band size: 42 kDaObserved band size: 45-60 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: JF1-045
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

EMMPRIN/CD147 Antibody (JF1-045) Summary

• Description: Novus Biologicals Rabbit EMMPRIN/CD147 Antibody (JF1-045) (NBP2-66820) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human EMMPRIN/CD147 aa 171-204 / 385. (SwissProt: P35613 Human; SwissProt: P18572 Mouse; SwissProt: P26453 Rat)
• Localization: Cell membrane, Melanosome.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BSG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:5,000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for EMMPRIN/CD147 Antibody (JF1-045)

• 5F7
• basigin (Ok blood group)
• Basigin
• BSG
• CD147 antigen
• CD147
• Collagenase stimulatory factor
• EMMPRIN
• EMMPRINTCSF
• Extracellular matrix metalloproteinase inducer
• Leukocyte activation antigen M6
• M6
• OK blood group antigen
• OK
• Tumor cell-derived collagenase stimulatory factor

Background

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for EMMPRIN/CD147 Antibody (NBP2-66820) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 03/22/2021

Summary

• Application: Immunocytochemistry
• Sample Tested: Human eye cells,Human eye
• Species: Human
• Lot: HN0727

Comments

• Comments: Use at 1:50 (overnight at 4 degrees)

Bioinformatics

• Gene Symbol: BSG