EMI1 Antibody (JG35-83)

Immunocytochemistry/Immunofluorescence: EMI1 Antibody (JG35-83) [NBP2-76833] - ICC staining Emi1 in Siha cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: EMI1 Antibody (JG35-83) [NBP2-76833] - Analysis of paraffin-embedded human colon carcinoma with Rabbit anti-EMI1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/Immunofluorescence: EMI1 Antibody (JG35-83) [NBP2-76833] - ICC staining Emi1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: EMI1 Antibody (JG35-83) [NBP2-76833] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Emi1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: EMI1 Antibody (JG35-83) [NBP2-76833] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Emi1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: EMI1 Antibody (JG35-83) [NBP2-76833] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-Emi1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: EMI1 Antibody (JG35-83) [NBP2-76833] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Emi1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: EMI1 Antibody (JG35-83) [NBP2-76833] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Emi1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: EMI1 Antibody (JG35-83) [NBP2-76833] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-EMI1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: EMI1 Antibody (JG35-83) [NBP2-76833] - Analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-EMI1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: EMI1 Antibody (JG35-83) [NBP2-76833] - Analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-EMI1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: EMI1 Antibody (JG35-83) [NBP2-76833] - Analysis of paraffin-embedded Human kidney with Rabbit anti-EMI1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

EMI1 Antibody (JG35-83) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human EMI1 aa 128-244 / 447. (SwissProt: Q9UKT4 Human; SwissProt: Q7TSG3 Mouse; SwissProt: B0BNA4 Rat)
• Localization: Cytoplasm. Cytoskeleton. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FBXO5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for EMI1 Antibody (JG35-83)

• EMI1Early mitotic inhibitor 1
• F-box only protein 5
• F-box protein 5
• FBX5F-box protein Fbx5
• Fbxo31

Background

Fbp5A is a member of the F-box protein family that is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to Xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase-promoting complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EMI1 Antibody (NBP2-76833)(1)

Publication using NBP2-76833

• Kang JW, Zhan Z, Ji G et al. PUMA facilitates EMI1-promoted cytoplasmic Rad51 ubiquitination and inhibits DNA repair in stem and progenitor cells Signal transduction and targeted therapy 2021-03-31 [PMID: 33785736] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: FBXO5